A comprehensive library of fluorescent constructs of SARS-CoV-2 proteins and their initial characterisation in different cell types

Stéphanie Miserey-Lenkei; Katarina Trajkovic; Juan Martín D'Ambrosio; Amanda J. Patel; Alenka Čopič; Pallavi Mathur; Kristine Schauer; Bruno Goud; Véronique Albanèse; Romain Gautier; Melody Subra; David Kovacs; Hélène Barelli; Bruno Antonny

doi:10.1111/boc.202000158

A comprehensive library of fluorescent constructs of SARS-CoV-2 proteins and their initial characterisation in different cell types

Stéphanie Miserey-Lenkei, Katarina Trajkovic, Juan Martín D'Ambrosio, Amanda J. Patel, Alenka Čopič, Pallavi Mathur, Kristine Schauer, Bruno Goud, Véronique Albanèse, Romain Gautier, Melody Subra, David Kovacs, Hélène Barelli, Bruno Antonny

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

Background Information: Comprehensive libraries of plasmids for SARS-CoV-2 proteins with various tags (e.g., Strep, HA, Turbo) are now available. They enable the identification of numerous potential protein–protein interactions between the SARS-CoV-2 virus and host proteins. Results: We present here a large library of SARS CoV-2 protein constructs fused with green and red fluorescent proteins and their initial characterisation in various human cell lines including lung epithelial cell models (A549, BEAS-2B), as well as in budding yeast. The localisation of a few SARS-CoV-2 proteins matches their proposed interactions with host proteins. These include the localisation of Nsp13 to the centrosome, Orf3a to late endosomes and Orf9b to mitochondria. Conclusions and Significance: This library should facilitate further cellular investigations, notably by imaging techniques.

Original language	English
Pages (from-to)	311-328
Number of pages	18
Journal	Biology of the Cell
Volume	113
Issue number	7
DOIs	https://doi.org/10.1111/boc.202000158
Publication status	Published - 1 Jul 2021
Externally published	Yes

Keywords

Intracellular compartmentalisation
Light microscopy
Membranes
Molecular interactions
Viruses

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10.1111/boc.202000158

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Miserey-Lenkei, S., Trajkovic, K., D'Ambrosio, J. M., Patel, A. J., Čopič, A., Mathur, P., Schauer, K., Goud, B., Albanèse, V., Gautier, R., Subra, M., Kovacs, D., Barelli, H., & Antonny, B. (2021). A comprehensive library of fluorescent constructs of SARS-CoV-2 proteins and their initial characterisation in different cell types. Biology of the Cell, 113(7), 311-328. https://doi.org/10.1111/boc.202000158

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title = "A comprehensive library of fluorescent constructs of SARS-CoV-2 proteins and their initial characterisation in different cell types",

abstract = "Background Information: Comprehensive libraries of plasmids for SARS-CoV-2 proteins with various tags (e.g., Strep, HA, Turbo) are now available. They enable the identification of numerous potential protein–protein interactions between the SARS-CoV-2 virus and host proteins. Results: We present here a large library of SARS CoV-2 protein constructs fused with green and red fluorescent proteins and their initial characterisation in various human cell lines including lung epithelial cell models (A549, BEAS-2B), as well as in budding yeast. The localisation of a few SARS-CoV-2 proteins matches their proposed interactions with host proteins. These include the localisation of Nsp13 to the centrosome, Orf3a to late endosomes and Orf9b to mitochondria. Conclusions and Significance: This library should facilitate further cellular investigations, notably by imaging techniques.",

keywords = "Intracellular compartmentalisation, Light microscopy, Membranes, Molecular interactions, Viruses",

author = "St{\'e}phanie Miserey-Lenkei and Katarina Trajkovic and D'Ambrosio, {Juan Mart{\'i}n} and Patel, {Amanda J.} and Alenka {\v C}opi{\v c} and Pallavi Mathur and Kristine Schauer and Bruno Goud and V{\'e}ronique Alban{\`e}se and Romain Gautier and Melody Subra and David Kovacs and H{\'e}l{\`e}ne Barelli and Bruno Antonny",

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Miserey-Lenkei, S, Trajkovic, K, D'Ambrosio, JM, Patel, AJ, Čopič, A, Mathur, P, Schauer, K, Goud, B, Albanèse, V, Gautier, R, Subra, M, Kovacs, D, Barelli, H & Antonny, B 2021, 'A comprehensive library of fluorescent constructs of SARS-CoV-2 proteins and their initial characterisation in different cell types', Biology of the Cell, vol. 113, no. 7, pp. 311-328. https://doi.org/10.1111/boc.202000158

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T1 - A comprehensive library of fluorescent constructs of SARS-CoV-2 proteins and their initial characterisation in different cell types

AU - Miserey-Lenkei, Stéphanie

AU - Trajkovic, Katarina

AU - D'Ambrosio, Juan Martín

AU - Patel, Amanda J.

AU - Čopič, Alenka

AU - Mathur, Pallavi

AU - Schauer, Kristine

AU - Goud, Bruno

AU - Albanèse, Véronique

AU - Gautier, Romain

AU - Subra, Melody

AU - Kovacs, David

AU - Barelli, Hélène

AU - Antonny, Bruno

PY - 2021/7/1

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AB - Background Information: Comprehensive libraries of plasmids for SARS-CoV-2 proteins with various tags (e.g., Strep, HA, Turbo) are now available. They enable the identification of numerous potential protein–protein interactions between the SARS-CoV-2 virus and host proteins. Results: We present here a large library of SARS CoV-2 protein constructs fused with green and red fluorescent proteins and their initial characterisation in various human cell lines including lung epithelial cell models (A549, BEAS-2B), as well as in budding yeast. The localisation of a few SARS-CoV-2 proteins matches their proposed interactions with host proteins. These include the localisation of Nsp13 to the centrosome, Orf3a to late endosomes and Orf9b to mitochondria. Conclusions and Significance: This library should facilitate further cellular investigations, notably by imaging techniques.

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JO - Biology of the Cell

JF - Biology of the Cell

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ER -

A comprehensive library of fluorescent constructs of SARS-CoV-2 proteins and their initial characterisation in different cell types

Abstract

Keywords

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