A single-cell sequencing guide for immunologists

Peter See, Josephine Lum, Jinmiao Chen, Florent Ginhoux

Research output: Contribution to journalReview articlepeer-review

153 Citations (Scopus)

Abstract

In recent years there has been a rapid increase in the use of single-cell sequencing (scRNA-seq) approaches in the field of immunology. With the wide range of technologies available, it is becoming harder for users to select the best scRNA-seq protocol/platform to address their biological questions of interest. Here, we compared the advantages and limitations of four commonly used scRNA-seq platforms in order to clarify their suitability for different experimental applications. We also address how the datasets generated by different scRNA-seq platforms can be integrated, and how to identify unknown populations of single cells using unbiased bioinformatics methods.

Original languageEnglish
Article number2425
JournalFrontiers in Immunology
Volume9
Issue numberOCT
DOIs
Publication statusPublished - 23 Oct 2018
Externally publishedYes

Keywords

  • 10X genomics chromium
  • Dendritic cells
  • Fluidigm C1
  • Immunology
  • MARS-seq
  • SMART-seq
  • Single-cell RNA sequencing

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