An AIF orthologue regulates apoptosis in yeast

Silke Wissing; Paula Ludovico; Eva Herker; Sabrina Büttner; Silvia M. Engelhardt; Thorsten Decker; Alexander Link; Astrid Proksch; Fernando Rodrigues; Manuela Corte-Real; Kai Uwe Fröhlich; Joachim Manns; Céline Candé; Stephan J. Sigrist; Guido Kroemer; Frank Madeo

doi:10.1083/jcb.200404138

An AIF orthologue regulates apoptosis in yeast

Silke Wissing, Paula Ludovico, Eva Herker, Sabrina Büttner, Silvia M. Engelhardt, Thorsten Decker, Alexander Link, Astrid Proksch, Fernando Rodrigues, Manuela Corte-Real, Kai Uwe Fröhlich, Joachim Manns, Céline Candé, Stephan J. Sigrist, Guido Kroemer, Frank Madeo

Research output: Contribution to journal › Article › peer-review

339 Citations (Scopus)

Abstract

Apoptosis-inducing factor (AIF), a key regulator of cell death, is essential for normal mammalian development and participates in pathological apoptosis. The proapoptotic nature of AIF and its mode of action are controversial. Here, we show that the yeast AIF homologue Ynr074cp controls yeast apoptosis. Similar to mammalian AIF, Ynr074cp is located in mitochondria and translocates to the nucleus of yeast cells in response to apoptotic stimuli. Purified Ynr074cp degrades yeast nuclei and plasmid DNA. YNR074C disruption rescues yeast cells from oxygen stress and delays age-induced apoptosis. Conversely, overexpression of Ynr074cp strongly stimulates apoptotic cell death induced by hydrogen peroxide and this effect is attenuated by disruption of cyclophilin A or the yeast caspase YCA1. We conclude that Ynr074cp is a cell death effector in yeast and rename it AIF-1 (Aif1p, gene AIF1).

Original language	English
Pages (from-to)	969-974
Number of pages	6
Journal	Journal of Cell Biology
Volume	166
Issue number	7
DOIs	https://doi.org/10.1083/jcb.200404138
Publication status	Published - 27 Sept 2004

Keywords

Aging
Apoptosis-inducing factor
Cyclophilin A
YCA1
YNR074C

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10.1083/jcb.200404138

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title = "An AIF orthologue regulates apoptosis in yeast",

abstract = "Apoptosis-inducing factor (AIF), a key regulator of cell death, is essential for normal mammalian development and participates in pathological apoptosis. The proapoptotic nature of AIF and its mode of action are controversial. Here, we show that the yeast AIF homologue Ynr074cp controls yeast apoptosis. Similar to mammalian AIF, Ynr074cp is located in mitochondria and translocates to the nucleus of yeast cells in response to apoptotic stimuli. Purified Ynr074cp degrades yeast nuclei and plasmid DNA. YNR074C disruption rescues yeast cells from oxygen stress and delays age-induced apoptosis. Conversely, overexpression of Ynr074cp strongly stimulates apoptotic cell death induced by hydrogen peroxide and this effect is attenuated by disruption of cyclophilin A or the yeast caspase YCA1. We conclude that Ynr074cp is a cell death effector in yeast and rename it AIF-1 (Aif1p, gene AIF1).",

keywords = "Aging, Apoptosis-inducing factor, Cyclophilin A, YCA1, YNR074C",

author = "Silke Wissing and Paula Ludovico and Eva Herker and Sabrina B{\"u}ttner and Engelhardt, {Silvia M.} and Thorsten Decker and Alexander Link and Astrid Proksch and Fernando Rodrigues and Manuela Corte-Real and Fr{\"o}hlich, {Kai Uwe} and Joachim Manns and C{\'e}line Cand{\'e} and Sigrist, {Stephan J.} and Guido Kroemer and Frank Madeo",

year = "2004",

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day = "27",

doi = "10.1083/jcb.200404138",

language = "English",

volume = "166",

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T1 - An AIF orthologue regulates apoptosis in yeast

AU - Wissing, Silke

AU - Ludovico, Paula

AU - Herker, Eva

AU - Büttner, Sabrina

AU - Engelhardt, Silvia M.

AU - Decker, Thorsten

AU - Link, Alexander

AU - Proksch, Astrid

AU - Rodrigues, Fernando

AU - Corte-Real, Manuela

AU - Fröhlich, Kai Uwe

AU - Manns, Joachim

AU - Candé, Céline

AU - Sigrist, Stephan J.

AU - Kroemer, Guido

AU - Madeo, Frank

PY - 2004/9/27

Y1 - 2004/9/27

N2 - Apoptosis-inducing factor (AIF), a key regulator of cell death, is essential for normal mammalian development and participates in pathological apoptosis. The proapoptotic nature of AIF and its mode of action are controversial. Here, we show that the yeast AIF homologue Ynr074cp controls yeast apoptosis. Similar to mammalian AIF, Ynr074cp is located in mitochondria and translocates to the nucleus of yeast cells in response to apoptotic stimuli. Purified Ynr074cp degrades yeast nuclei and plasmid DNA. YNR074C disruption rescues yeast cells from oxygen stress and delays age-induced apoptosis. Conversely, overexpression of Ynr074cp strongly stimulates apoptotic cell death induced by hydrogen peroxide and this effect is attenuated by disruption of cyclophilin A or the yeast caspase YCA1. We conclude that Ynr074cp is a cell death effector in yeast and rename it AIF-1 (Aif1p, gene AIF1).

AB - Apoptosis-inducing factor (AIF), a key regulator of cell death, is essential for normal mammalian development and participates in pathological apoptosis. The proapoptotic nature of AIF and its mode of action are controversial. Here, we show that the yeast AIF homologue Ynr074cp controls yeast apoptosis. Similar to mammalian AIF, Ynr074cp is located in mitochondria and translocates to the nucleus of yeast cells in response to apoptotic stimuli. Purified Ynr074cp degrades yeast nuclei and plasmid DNA. YNR074C disruption rescues yeast cells from oxygen stress and delays age-induced apoptosis. Conversely, overexpression of Ynr074cp strongly stimulates apoptotic cell death induced by hydrogen peroxide and this effect is attenuated by disruption of cyclophilin A or the yeast caspase YCA1. We conclude that Ynr074cp is a cell death effector in yeast and rename it AIF-1 (Aif1p, gene AIF1).

KW - Aging

KW - Apoptosis-inducing factor

KW - Cyclophilin A

KW - YCA1

KW - YNR074C

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U2 - 10.1083/jcb.200404138

DO - 10.1083/jcb.200404138

M3 - Article

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AN - SCOPUS:4644246439

SN - 0021-9525

VL - 166

SP - 969

EP - 974

JO - Journal of Cell Biology

JF - Journal of Cell Biology

IS - 7

ER -

An AIF orthologue regulates apoptosis in yeast

Abstract

Keywords

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