TY - JOUR
T1 - Breakpoint Heterogeneity in t(10;11) Translocation in AML-M4/M5 Resulting in Fusion of AF10 and MLL Is Resolved by Fluorescent in Situ Hybridization Analysis
AU - Beverloo, H. Berna
AU - Wijsman, Jacqueline
AU - de Klein, Annelies
AU - Hagemeijer, Anne
AU - Coniat, Maryvonne Le
AU - Bernard, Olivier
AU - Berger, Roland
AU - Lillington, Debra M.
AU - Young, Bryan D.
AU - van Wering, Elisabeth
AU - Welborn, Jeanna
PY - 1995/10/1
Y1 - 1995/10/1
N2 - Ten AML-M4/M5 patients’ samples containing a t(10;11) translocation, but with different cytogenetic breakpoints on chromosome 11q (11ql3-23), were studied by G- and R-banding and fluorescent in situ hybridization. Southern blotting analysis, studied in five patients, revealed a rearranged MLL gene. Reverse transcription-PCR analysis carried out in six patients showed a 5’ MLL-3’ AF-10 fusion transcript Fluorescent in situ hybridization studies suggested that in 8 of 10 patients, the rearrangement/fusion transcript resulted from an inversion of a part of 11q (ql3q23) translocated to 10pl2. In the other two patients, it is assumed that an inversion/translocation has occurred of a part of lOp to the der(ll). The results suggest that the orientation of the AF-10 gene on lOp is 5’ telomeric and 3’ centromeric This is the first example of opposite-oriented genes being involved in translocation to yield fusion transcripts.
AB - Ten AML-M4/M5 patients’ samples containing a t(10;11) translocation, but with different cytogenetic breakpoints on chromosome 11q (11ql3-23), were studied by G- and R-banding and fluorescent in situ hybridization. Southern blotting analysis, studied in five patients, revealed a rearranged MLL gene. Reverse transcription-PCR analysis carried out in six patients showed a 5’ MLL-3’ AF-10 fusion transcript Fluorescent in situ hybridization studies suggested that in 8 of 10 patients, the rearrangement/fusion transcript resulted from an inversion of a part of 11q (ql3q23) translocated to 10pl2. In the other two patients, it is assumed that an inversion/translocation has occurred of a part of lOp to the der(ll). The results suggest that the orientation of the AF-10 gene on lOp is 5’ telomeric and 3’ centromeric This is the first example of opposite-oriented genes being involved in translocation to yield fusion transcripts.
UR - http://www.scopus.com/inward/record.url?scp=0029068727&partnerID=8YFLogxK
M3 - Article
C2 - 7671224
AN - SCOPUS:0029068727
SN - 0008-5472
VL - 55
SP - 4220
EP - 4224
JO - Cancer Research
JF - Cancer Research
IS - 19
ER -