Building consensus spectral libraries for peptide identification in proteomics

Henry Lam; Eric W. Deutsch; James S. Eddes; Jimmy K. Eng; Stephen E. Stein; Ruedi Aebersold

doi:10.1038/nmeth.1254

Building consensus spectral libraries for peptide identification in proteomics

Henry Lam, Eric W. Deutsch, James S. Eddes, Jimmy K. Eng, Stephen E. Stein, Ruedi Aebersold

Research output: Contribution to journal › Article › peer-review

236 Citations (Scopus)

Abstract

Spectral searching has drawn increasing interest as an alternative to sequence-database searching in proteomics. We developed and validated an open-source software toolkit, SpectraST, to enable proteomics researchers to build spectral libraries and to integrate this promising approach in their data-analysis pipeline. It allows individual researchers to condense raw data into spectral libraries, summarizing information about observed proteomes into a concise and retrievable format for future data analyses.

Original language	English
Pages (from-to)	873-875
Number of pages	3
Journal	Nature Methods
Volume	5
Issue number	10
DOIs	https://doi.org/10.1038/nmeth.1254
Publication status	Published - 22 Sept 2008
Externally published	Yes

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10.1038/nmeth.1254

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abstract = "Spectral searching has drawn increasing interest as an alternative to sequence-database searching in proteomics. We developed and validated an open-source software toolkit, SpectraST, to enable proteomics researchers to build spectral libraries and to integrate this promising approach in their data-analysis pipeline. It allows individual researchers to condense raw data into spectral libraries, summarizing information about observed proteomes into a concise and retrievable format for future data analyses.",

author = "Henry Lam and Deutsch, {Eric W.} and Eddes, {James S.} and Eng, {Jimmy K.} and Stein, {Stephen E.} and Ruedi Aebersold",

note = "Funding Information: We thank all the data contributors to PeptideAtlas who have made this study possible. This study was supported in part by the US National Heart, Lung and Blood Institute, National Institutes of Health (N01-HV-28179).",

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AU - Aebersold, Ruedi

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Building consensus spectral libraries for peptide identification in proteomics

Abstract

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