TY - JOUR
T1 - CD48 may serve as an accessory molecule for the activation of a subset of human γ/δ T cells
AU - Flament, Caroline
AU - Bellagha, Kamel
AU - Rosenthal-Allieri, Alexandra
AU - Chouaib, Salem
AU - Mami-Chouaib, Fathia
N1 - Funding Information:
F.M.C. is supported by a grant (No. 2036) from ARC (Asso-
PY - 1996/4/1
Y1 - 1996/4/1
N2 - To further assess the role of CD48 in the interaction of human γ/δ T cells with their specific target, we generated two series of alloreactive clones, L and K. These clones express a V1-D-J1-C δ chain associated to V3- J2-C2 (L) or V2-J2-C2 (K) γ chain. Functionally they were CTLs able to lyse the sensitizing B-cell line E418. The cytotoxicity of the L and K clones toward E418 was inhibited by anti-CD48 mAb. That of the L clones was also inhibited by anti-HLA class I mAbs. Variation in L and K lysis profile was observed against a panel of CD48+ targets, further strengthening the argument that they display distinct specificities and suggesting that they do not recognize CD48. Heterogeneity in TCR gene segment usage, MHC-dependent recognition of E418 by the L clones, and resistance of some CD48+ targets strongly suggest that CD48 itself does not interact with L and K TCR. Transfection of CHO cells with CD48 induced killing by the K clones. This killing was inhibited by anti-CD48 mAbs. Taking into account the recent reports on CD48 as an accessory molecule, our results suggest that by binding to CD2 (and/or an unknown ligand), CD48 may serve to strengthen E/T interaction and may contribute to the activation of a minor subset of γ/δ T cells.
AB - To further assess the role of CD48 in the interaction of human γ/δ T cells with their specific target, we generated two series of alloreactive clones, L and K. These clones express a V1-D-J1-C δ chain associated to V3- J2-C2 (L) or V2-J2-C2 (K) γ chain. Functionally they were CTLs able to lyse the sensitizing B-cell line E418. The cytotoxicity of the L and K clones toward E418 was inhibited by anti-CD48 mAb. That of the L clones was also inhibited by anti-HLA class I mAbs. Variation in L and K lysis profile was observed against a panel of CD48+ targets, further strengthening the argument that they display distinct specificities and suggesting that they do not recognize CD48. Heterogeneity in TCR gene segment usage, MHC-dependent recognition of E418 by the L clones, and resistance of some CD48+ targets strongly suggest that CD48 itself does not interact with L and K TCR. Transfection of CHO cells with CD48 induced killing by the K clones. This killing was inhibited by anti-CD48 mAbs. Taking into account the recent reports on CD48 as an accessory molecule, our results suggest that by binding to CD2 (and/or an unknown ligand), CD48 may serve to strengthen E/T interaction and may contribute to the activation of a minor subset of γ/δ T cells.
UR - http://www.scopus.com/inward/record.url?scp=0029889550&partnerID=8YFLogxK
U2 - 10.1016/0198-8859(96)00010-9
DO - 10.1016/0198-8859(96)00010-9
M3 - Article
C2 - 8727206
AN - SCOPUS:0029889550
SN - 0198-8859
VL - 46
SP - 82
EP - 92
JO - Human Immunology
JF - Human Immunology
IS - 2
ER -