Characterization of Developmental Trajectories of Dendritic Cell Hematopoiesis Through Single-Cell RNA Sequencing Methods

Regine J. Dress, Florent Ginhoux

    Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

    1 Citation (Scopus)

    Abstract

    Single-cell technologies have become valuable tools to trace dendritic cell differentiation trajectories. Here, we illustrate the workflow used for processing of mouse bone marrow for single-cell RNA sequencing and trajectory analyses, as done in Dress et al. (Nat Immunol 20:852–864, 2019). This short methodology is presented as a starting point for researchers just beginning to dive into the complex field of dendritic cell ontogeny and cellular development trajectory analyses.

    Original languageEnglish
    Title of host publicationMethods in Molecular Biology
    PublisherHumana Press Inc.
    Pages375-385
    Number of pages11
    DOIs
    Publication statusPublished - 1 Jan 2023

    Publication series

    NameMethods in Molecular Biology
    Volume2618
    ISSN (Print)1064-3745
    ISSN (Electronic)1940-6029

    Keywords

    • DC
    • Dendritic cells
    • Hematopoiesis
    • Progenitor
    • RNA-seq
    • Single cell
    • Slingshot
    • Trajectory
    • UMAP
    • cMap

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