Conservation des propriétés allostériques de la protéine réceptrice de l'acétylcholine en solution détergente sans addition de lipides.

T. Heidmann; J. B. Cuisinier; J. P. Changeux

Conservation des propriétés allostériques de la protéine réceptrice de l'acétylcholine en solution détergente sans addition de lipides.

Translated title of the contribution: Preservation of the allosteric properties of the protein receptor for acetylcholine in detergent solution without addition of lipids

T. Heidmann, J. B. Cuisinier, J. P. Changeux

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Solubilization by Na cholate of acetylcholine receptor-rich membrane fragments from Torpedo marmorata, followed by exchange of NA cholate by the neutral detergent "Tween 80" yields the receptor protein in its 9 S soluble light form; under these conditions, without adding lipids, the receptor protein conserves its characteristic binding properties for the fluorescent agonist Dns-C6-Cho as followed by fast kinetic techniques, and the allosteric regulation by non-competitive blockers.

Translated title of the contribution	Preservation of the allosteric properties of the protein receptor for acetylcholine in detergent solution without addition of lipids
Original language	French
Pages (from-to)	13-15
Number of pages	3
Journal	Comptes Rendus de l'Academie des Sciences - Serie III
Volume	292
Issue number	1
Publication status	Published - 5 Jan 1981

Cite this

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title = "Conservation des propri{\'e}t{\'e}s allost{\'e}riques de la prot{\'e}ine r{\'e}ceptrice de l'ac{\'e}tylcholine en solution d{\'e}tergente sans addition de lipides.",

abstract = "Solubilization by Na cholate of acetylcholine receptor-rich membrane fragments from Torpedo marmorata, followed by exchange of NA cholate by the neutral detergent {"}Tween 80{"} yields the receptor protein in its 9 S soluble light form; under these conditions, without adding lipids, the receptor protein conserves its characteristic binding properties for the fluorescent agonist Dns-C6-Cho as followed by fast kinetic techniques, and the allosteric regulation by non-competitive blockers.",

author = "T. Heidmann and Cuisinier, {J. B.} and Changeux, {J. P.}",

year = "1981",

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language = "Fran{\c c}ais",

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journal = "Comptes Rendus de l'Academie des Sciences - Serie III",

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AU - Heidmann, T.

AU - Cuisinier, J. B.

AU - Changeux, J. P.

PY - 1981/1/5

Y1 - 1981/1/5

N2 - Solubilization by Na cholate of acetylcholine receptor-rich membrane fragments from Torpedo marmorata, followed by exchange of NA cholate by the neutral detergent "Tween 80" yields the receptor protein in its 9 S soluble light form; under these conditions, without adding lipids, the receptor protein conserves its characteristic binding properties for the fluorescent agonist Dns-C6-Cho as followed by fast kinetic techniques, and the allosteric regulation by non-competitive blockers.

AB - Solubilization by Na cholate of acetylcholine receptor-rich membrane fragments from Torpedo marmorata, followed by exchange of NA cholate by the neutral detergent "Tween 80" yields the receptor protein in its 9 S soluble light form; under these conditions, without adding lipids, the receptor protein conserves its characteristic binding properties for the fluorescent agonist Dns-C6-Cho as followed by fast kinetic techniques, and the allosteric regulation by non-competitive blockers.

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AN - SCOPUS:0010263615

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JO - Comptes Rendus de l'Academie des Sciences - Serie III

JF - Comptes Rendus de l'Academie des Sciences - Serie III

IS - 1

ER -

Conservation des propriétés allostériques de la protéine réceptrice de l'acétylcholine en solution détergente sans addition de lipides.

Abstract

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