TY - JOUR
T1 - Decreased DNA-PK activity in human cancer cells exhibiting hypersensitivity to low-dose irradiation
AU - Vaganay-Juéry, S.
AU - Muller, C.
AU - Marangoni, E.
AU - Abdulkarim, B.
AU - Deutsch, E.
AU - Lambin, P.
AU - Calsou, P.
AU - Eschwege, F.
AU - Salles, B.
AU - Joiner, M.
AU - Bourhis, J.
N1 - Funding Information:
This work was supported in part by Ligue Nationale Contre le Cancer, Association pour la Recherche sur le Cancer ARC 96–92, Institut Fédératif de Recherche, IFR No 54, Institut Gustave Roussy (Bourse ‘Project IFR’, 1998–99). We gratefully thank Nicolas Foray PhD for discussions.
PY - 2000/1/1
Y1 - 2000/1/1
N2 - Low-dose hyper-radiosensitivity (HRS) (below 0.5 Gy) has been extensively documented in the past few years. The molecular basis of this phenomenon remains largely unknown and the purpose of this study was to investigate the possible implication of the DNA repair DNA-PK complex. The activity of the DNA-PK complex, i.e. Ku DNA-end binding activity and kinase activity of the whole complex, was studied in 10 human cancer cell lines, 2 h after 0.2, 0.5 and 1 Gy irradiation. After low-dose irradiation (0.2 Gy), a marked decrease in DNA-PK activity was found in all six cell lines exhibiting HRS, whereas the DNA-PK activity was increased in the four cell lines which did not exhibit HRS. This modulation of DNA-PK activity was a rapid phenomenon occurring within the 2 h following low-dose radiation exposure. These data strongly suggest the implication of the DNA-PK repair complex in the HRS phenomenon. (C) 2000 Cancer Research Campaign.
AB - Low-dose hyper-radiosensitivity (HRS) (below 0.5 Gy) has been extensively documented in the past few years. The molecular basis of this phenomenon remains largely unknown and the purpose of this study was to investigate the possible implication of the DNA repair DNA-PK complex. The activity of the DNA-PK complex, i.e. Ku DNA-end binding activity and kinase activity of the whole complex, was studied in 10 human cancer cell lines, 2 h after 0.2, 0.5 and 1 Gy irradiation. After low-dose irradiation (0.2 Gy), a marked decrease in DNA-PK activity was found in all six cell lines exhibiting HRS, whereas the DNA-PK activity was increased in the four cell lines which did not exhibit HRS. This modulation of DNA-PK activity was a rapid phenomenon occurring within the 2 h following low-dose radiation exposure. These data strongly suggest the implication of the DNA-PK repair complex in the HRS phenomenon. (C) 2000 Cancer Research Campaign.
KW - DNA repair
KW - DNA-PK
KW - Ionizing radiation
KW - Low-dose hyper-radiosensitivity
UR - http://www.scopus.com/inward/record.url?scp=0033915850&partnerID=8YFLogxK
U2 - 10.1054/bjoc.2000.1258
DO - 10.1054/bjoc.2000.1258
M3 - Article
C2 - 10945500
AN - SCOPUS:0033915850
SN - 0007-0920
VL - 83
SP - 514
EP - 518
JO - British Journal of Cancer
JF - British Journal of Cancer
IS - 4
ER -