Detection and quantification of minority KRAS subclones in tumors using droplet-based microfluidics: Clinical implication

D. Pekin; C. Normand; S. Kotsopoulos; X. Li; L. Benhaim; O. Bouché; T. Lecompte; D. Le Corre; T. Hor; Z. El Harrak; P. Nizard; D. Link; J. B. Hutschinson; P. Laurent-Puig; V. Taly

Detection and quantification of minority KRAS subclones in tumors using droplet-based microfluidics: Clinical implication

D. Pekin, C. Normand, S. Kotsopoulos, X. Li, L. Benhaim, O. Bouché, T. Lecompte, D. Le Corre, T. Hor, Z. El Harrak, P. Nizard, D. Link, J. B. Hutschinson, P. Laurent-Puig, V. Taly

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

We used a multiplex droplet-based digital PCR method in a retrospective study including 181 patients with a metastatic colorectal cancer (mCRC) for the quantitative and sensitive detection of KRAS and BRAF mutations in DNA extracted from tumors.

Original language	English
Title of host publication	17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Publisher	Chemical and Biological Microsystems Society
Pages	1806-1808
Number of pages	3
ISBN (Print)	9781632666246
Publication status	Published - 1 Jan 2013
Externally published	Yes
Event	17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany Duration: 27 Oct 2013 → 31 Oct 2013

Publication series

Name	17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Volume	3

Conference

Conference	17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Country/Territory	Germany
City	Freiburg
Period	27/10/13 → 31/10/13

Keywords

Cancer biomarkers
Digital PCR (dPCR)
Droplet-based microfluidics
KRAS mutations

Cite this

Pekin, D., Normand, C., Kotsopoulos, S., Li, X., Benhaim, L., Bouché, O., Lecompte, T., Le Corre, D., Hor, T., El Harrak, Z., Nizard, P., Link, D., Hutschinson, J. B., Laurent-Puig, P., & Taly, V. (2013). Detection and quantification of minority KRAS subclones in tumors using droplet-based microfluidics: Clinical implication. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 (pp. 1806-1808). (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Vol. 3). Chemical and Biological Microsystems Society.

Pekin, D. ; Normand, C. ; Kotsopoulos, S. et al. / Detection and quantification of minority KRAS subclones in tumors using droplet-based microfluidics : Clinical implication. 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Chemical and Biological Microsystems Society, 2013. pp. 1806-1808 (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013).

@inproceedings{e2dc37548dd6453187d561b5521bcd91,

title = "Detection and quantification of minority KRAS subclones in tumors using droplet-based microfluidics: Clinical implication",

abstract = "We used a multiplex droplet-based digital PCR method in a retrospective study including 181 patients with a metastatic colorectal cancer (mCRC) for the quantitative and sensitive detection of KRAS and BRAF mutations in DNA extracted from tumors.",

keywords = "Cancer biomarkers, Digital PCR (dPCR), Droplet-based microfluidics, KRAS mutations",

author = "D. Pekin and C. Normand and S. Kotsopoulos and X. Li and L. Benhaim and O. Bouch{\'e} and T. Lecompte and {Le Corre}, D. and T. Hor and {El Harrak}, Z. and P. Nizard and D. Link and Hutschinson, {J. B.} and P. Laurent-Puig and V. Taly",

year = "2013",

month = jan,

day = "1",

language = "English",

isbn = "9781632666246",

series = "17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013",

publisher = "Chemical and Biological Microsystems Society",

pages = "1806--1808",

booktitle = "17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013",

note = "17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 ; Conference date: 27-10-2013 Through 31-10-2013",

}

Pekin, D, Normand, C, Kotsopoulos, S, Li, X, Benhaim, L, Bouché, O, Lecompte, T, Le Corre, D, Hor, T, El Harrak, Z, Nizard, P, Link, D, Hutschinson, JB, Laurent-Puig, P & Taly, V 2013, Detection and quantification of minority KRAS subclones in tumors using droplet-based microfluidics: Clinical implication. in 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, vol. 3, Chemical and Biological Microsystems Society, pp. 1806-1808, 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, Freiburg, Germany, 27/10/13.

Detection and quantification of minority KRAS subclones in tumors using droplet-based microfluidics: Clinical implication. / Pekin, D.; Normand, C.; Kotsopoulos, S. et al.
17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Chemical and Biological Microsystems Society, 2013. p. 1806-1808 (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Vol. 3).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Detection and quantification of minority KRAS subclones in tumors using droplet-based microfluidics

T2 - 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013

AU - Pekin, D.

AU - Normand, C.

AU - Kotsopoulos, S.

AU - Li, X.

AU - Benhaim, L.

AU - Bouché, O.

AU - Lecompte, T.

AU - Le Corre, D.

AU - Hor, T.

AU - El Harrak, Z.

AU - Nizard, P.

AU - Link, D.

AU - Hutschinson, J. B.

AU - Laurent-Puig, P.

AU - Taly, V.

PY - 2013/1/1

Y1 - 2013/1/1

N2 - We used a multiplex droplet-based digital PCR method in a retrospective study including 181 patients with a metastatic colorectal cancer (mCRC) for the quantitative and sensitive detection of KRAS and BRAF mutations in DNA extracted from tumors.

AB - We used a multiplex droplet-based digital PCR method in a retrospective study including 181 patients with a metastatic colorectal cancer (mCRC) for the quantitative and sensitive detection of KRAS and BRAF mutations in DNA extracted from tumors.

KW - Cancer biomarkers

KW - Digital PCR (dPCR)

KW - Droplet-based microfluidics

KW - KRAS mutations

UR - http://www.scopus.com/inward/record.url?scp=84907372240&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:84907372240

SN - 9781632666246

T3 - 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013

SP - 1806

EP - 1808

BT - 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013

PB - Chemical and Biological Microsystems Society

Y2 - 27 October 2013 through 31 October 2013

ER -

Pekin D, Normand C, Kotsopoulos S, Li X, Benhaim L, Bouché O et al. Detection and quantification of minority KRAS subclones in tumors using droplet-based microfluidics: Clinical implication. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Chemical and Biological Microsystems Society. 2013. p. 1806-1808. (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013).

Detection and quantification of minority KRAS subclones in tumors using droplet-based microfluidics: Clinical implication

Abstract

Publication series

Conference

Keywords

Other files and links

Cite this