Detection of gene rearrangements in circulating tumor cells: Examples of ALK-, ROS1-, RET-rearrangements in non-small-cell lung cancer and ERG-rearrangements in prostate cancer

Cyril Catelain, Emma Pailler, Marianne Oulhen, Vincent Faugeroux, Anne Laure Pommier, Françoise Farace

    Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

    14 Citations (Scopus)

    Abstract

    Circulating tumor cells (CTCs) hold promise as biomarkers to aid in patient treatment stratification and disease monitoring. Because the number of cells is a critical parameter for exploiting CTCs for predictive biomarker’s detection, we developed a FISH (fluorescent in situ hybridization) method for CTCs enriched on filters (filter-adapted FISH [FA-FISH]) that was optimized for high cell recovery. To increase the feasibility and reliability of the analyses, we combined fluorescent staining and FA-FISH and developed a semi-automated microscopy method for optimal FISH signal identification in filtration-enriched CTCs. Here we present these methods and their use for the detection and characterization of ALK-, ROS1-, RET-rearrangement in CTCs from non-small-cell lung cancer and ERG-rearrangements in CTCs from prostate cancer patients.

    Original languageEnglish
    Title of host publicationAdvances in Experimental Medicine and Biology
    PublisherSpringer New York LLC
    Pages169-179
    Number of pages11
    DOIs
    Publication statusPublished - 1 Jan 2017

    Publication series

    NameAdvances in Experimental Medicine and Biology
    Volume994
    ISSN (Print)0065-2598
    ISSN (Electronic)2214-8019

    Keywords

    • Circulating tumor cells
    • FA-FISH
    • Filtration-enrichment
    • Fluorescent staining
    • Predictive biomarkers

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