Detection of single-copy genes by nonisotopic in situ hybridization on human chromosomes

Dorra Cherif; Olivier Bernard; Roland Berger

doi:10.1007/BF00283691

Detection of single-copy genes by nonisotopic in situ hybridization on human chromosomes

Dorra Cherif, Olivier Bernard, Roland Berger

Research output: Contribution to journal › Article › peer-review

75 Citations (Scopus)

Abstract

A technique of in situ hybridization on metaphase chromosomes with biotinylated DNA probes is described. This technique was used to localize unique DNA sequences on chromosomes and allowed a localization of two probes 1.8 and 1.3 kb long. The hybridization signal appears like two, twin, spots on the two sister chromatids, allowing a clear distinction from the background. Moreover a chromosomal localization is possible by counting a relatively small number of mitoses compared with the technique using ³H-labeled DNA probes.

Original language	English
Pages (from-to)	358-362
Number of pages	5
Journal	Human Genetics
Volume	81
Issue number	4
DOIs	https://doi.org/10.1007/BF00283691
Publication status	Published - 1 Mar 1989
Externally published	Yes

Access to Document

10.1007/BF00283691

Cite this

@article{c7b6062b391d4c0e8ee103e1fbaa7203,

title = "Detection of single-copy genes by nonisotopic in situ hybridization on human chromosomes",

abstract = "A technique of in situ hybridization on metaphase chromosomes with biotinylated DNA probes is described. This technique was used to localize unique DNA sequences on chromosomes and allowed a localization of two probes 1.8 and 1.3 kb long. The hybridization signal appears like two, twin, spots on the two sister chromatids, allowing a clear distinction from the background. Moreover a chromosomal localization is possible by counting a relatively small number of mitoses compared with the technique using 3H-labeled DNA probes.",

author = "Dorra Cherif and Olivier Bernard and Roland Berger",

year = "1989",

month = mar,

day = "1",

doi = "10.1007/BF00283691",

language = "English",

volume = "81",

pages = "358--362",

journal = "Human Genetics",

issn = "0340-6717",

number = "4",

}

TY - JOUR

T1 - Detection of single-copy genes by nonisotopic in situ hybridization on human chromosomes

AU - Cherif, Dorra

AU - Bernard, Olivier

AU - Berger, Roland

PY - 1989/3/1

Y1 - 1989/3/1

N2 - A technique of in situ hybridization on metaphase chromosomes with biotinylated DNA probes is described. This technique was used to localize unique DNA sequences on chromosomes and allowed a localization of two probes 1.8 and 1.3 kb long. The hybridization signal appears like two, twin, spots on the two sister chromatids, allowing a clear distinction from the background. Moreover a chromosomal localization is possible by counting a relatively small number of mitoses compared with the technique using 3H-labeled DNA probes.

AB - A technique of in situ hybridization on metaphase chromosomes with biotinylated DNA probes is described. This technique was used to localize unique DNA sequences on chromosomes and allowed a localization of two probes 1.8 and 1.3 kb long. The hybridization signal appears like two, twin, spots on the two sister chromatids, allowing a clear distinction from the background. Moreover a chromosomal localization is possible by counting a relatively small number of mitoses compared with the technique using 3H-labeled DNA probes.

UR - http://www.scopus.com/inward/record.url?scp=0024512530&partnerID=8YFLogxK

U2 - 10.1007/BF00283691

DO - 10.1007/BF00283691

M3 - Article

C2 - 2703238

AN - SCOPUS:0024512530

SN - 0340-6717

VL - 81

SP - 358

EP - 362

JO - Human Genetics

JF - Human Genetics

IS - 4

ER -

Detection of single-copy genes by nonisotopic in situ hybridization on human chromosomes

Abstract

Access to Document

Other files and links

Cite this