TY - JOUR
T1 - DNA replication-dependent formation of joint DNA molecules in Physarum polycephalum
AU - Bénard, Marianne
AU - Maric, Chrystelle
AU - Pierron, Gérard
N1 - Funding Information:
We thank Steve West for kindly providing the RuvC enzyme, and Dominick Pallotta and Rodney Rothstein for critical reading of the manuscript. This work was supported by general funding from the CNRS and by a grant from Association pour la Recherche contre le Cancer. C.M. is a postdoctoral fellow of la Ligue Nationale contre le Cancer.
PY - 2001/5/25
Y1 - 2001/5/25
N2 - Two-dimensional neutral/neutral agarose gel electrophoresis is used extensively to localize replication origins. This method resolves DNA structures containing replication forks. It also detects X-shaped recombination intermediates in meiotic cells, in the form of a typical vertical spike. Intriguingly, such a spike of joint DNA molecules is often detectable in replicating DNA from mitotic cells. Here, we used naturally synchronous DNA samples from Physarum polycephalum to demonstrate that postreplicative, DNA replication-dependent X-shaped DNA molecules are formed between sister chromatids. These molecules have physical properties reminiscent of Holliday junctions. Our results demonstrate frequent interactions between sister chromatids during a normal cell cycle and suggest a novel phase during DNA replication consisting of transient, joint DNA molecules formed on newly replicated DNA.
AB - Two-dimensional neutral/neutral agarose gel electrophoresis is used extensively to localize replication origins. This method resolves DNA structures containing replication forks. It also detects X-shaped recombination intermediates in meiotic cells, in the form of a typical vertical spike. Intriguingly, such a spike of joint DNA molecules is often detectable in replicating DNA from mitotic cells. Here, we used naturally synchronous DNA samples from Physarum polycephalum to demonstrate that postreplicative, DNA replication-dependent X-shaped DNA molecules are formed between sister chromatids. These molecules have physical properties reminiscent of Holliday junctions. Our results demonstrate frequent interactions between sister chromatids during a normal cell cycle and suggest a novel phase during DNA replication consisting of transient, joint DNA molecules formed on newly replicated DNA.
UR - http://www.scopus.com/inward/record.url?scp=0035947237&partnerID=8YFLogxK
U2 - 10.1016/S1097-2765(01)00237-4
DO - 10.1016/S1097-2765(01)00237-4
M3 - Article
C2 - 11389844
AN - SCOPUS:0035947237
SN - 1097-2765
VL - 7
SP - 971
EP - 980
JO - Molecular Cell
JF - Molecular Cell
IS - 5
ER -