TY - JOUR
T1 - Establishment and characterization of new orthotopic and metastatic neuroblastoma models
AU - Daudigeos-Dubus, Estelle
AU - Le Dret, Ludivine
AU - Rouffiac, Valérie
AU - Bawa, Olivia
AU - Leguerney, Ingrid
AU - Opolon, Paule
AU - Vassal, Gilles
AU - Geoerger, Birgit
PY - 2014/7/1
Y1 - 2014/7/1
N2 - Background/Aim: Treatment of metastatic neuroblastoma remains a challenge in pediatric oncology. Relevant preclinical models may improve exploration of oncogenesis and new therapies. We developed new orthotopic and metastatic models derived from stage 4 neuroblastoma. Material and Methods: Orthotopic and systemic models were established in BalbC Rag2-/-gammaC-/- mice following adrenal and intravenous injection of luciferase-transfected IMR-32 and IGR-N91 cells, respectively. Results: All four models exhibited 100% tumor take rate. Metastatic spread of orthotopic IMR-32- Luc cells was observed mainly to the lung, liver and bone; that of IGR-N91-Luc cells to liver, spleen and adrenals. Interestingly, systemic IMR-32-Luc cells metastasized rather to the lung, liver and bone, and IGR-N91-Luc to liver, lung, spleen and adrenals. Feasibility of non-invasive, real-time antitumor response evaluation was validated in the systemic models. Conclusion: These neuroblastoma models with distinct patterns of metastatic spread represent relevant tools for exploring local and metastatic tumor cell tropism, mechanisms of spread and evaluating new cancer therapeutics.
AB - Background/Aim: Treatment of metastatic neuroblastoma remains a challenge in pediatric oncology. Relevant preclinical models may improve exploration of oncogenesis and new therapies. We developed new orthotopic and metastatic models derived from stage 4 neuroblastoma. Material and Methods: Orthotopic and systemic models were established in BalbC Rag2-/-gammaC-/- mice following adrenal and intravenous injection of luciferase-transfected IMR-32 and IGR-N91 cells, respectively. Results: All four models exhibited 100% tumor take rate. Metastatic spread of orthotopic IMR-32- Luc cells was observed mainly to the lung, liver and bone; that of IGR-N91-Luc cells to liver, spleen and adrenals. Interestingly, systemic IMR-32-Luc cells metastasized rather to the lung, liver and bone, and IGR-N91-Luc to liver, lung, spleen and adrenals. Feasibility of non-invasive, real-time antitumor response evaluation was validated in the systemic models. Conclusion: These neuroblastoma models with distinct patterns of metastatic spread represent relevant tools for exploring local and metastatic tumor cell tropism, mechanisms of spread and evaluating new cancer therapeutics.
KW - Bioluminescence imaging
KW - Metastasis pattern
KW - Neuroblastoma
KW - Orthotopic and systemic human xenograft model
UR - http://www.scopus.com/inward/record.url?scp=84918500855&partnerID=8YFLogxK
M3 - Article
C2 - 24982206
AN - SCOPUS:84918500855
SN - 0258-851X
VL - 28
SP - 425
EP - 434
JO - In Vivo
JF - In Vivo
IS - 4
ER -