TY - JOUR
T1 - Expression and proliferative effect of hemokinin-1 in human B-cells
AU - Grassin-Delyle, Stanislas
AU - Buenestado, Amparo
AU - Vallat, Laurent
AU - Naline, Emmanuel
AU - Marx, Sirima
AU - Decocq, Julie
AU - Debré, Patrice
AU - Bernard, Olivier A.
AU - Advenier, Charles
AU - Devillier, Philippe
AU - Merle-Béral, Hélne
PY - 2011/5/1
Y1 - 2011/5/1
N2 - Tachykinins are a family of structurally related peptides, including substance P (SP), hemokinin-1 (HK-1), neurokinin A (NKA), and neurokinin B. SP and NKA have been shown to modulate hematopoiesis and rat/mouse HK-1 has been found to be involved in the survival and differentiation of mouse B-cells. This study was designed to assess the expression of tachykinins with a focus on human HK-1 (hHK-1) in human B lymphocytes and the role of these peptides in cell differentiation, apoptosis and proliferation. Expression of tachykinin and tachykinin receptor mRNA was determined quantitatively in human B lymphoproliferative malignancies and compared to normal B-cells. Expression of hHK-1 and NK1 receptor, but not SP, was detected in human B-lymphocytes, and was up-regulated in B-lymphocytes from chronic lymphocytic leukemia and non-Hodgkin's lymphoma, while it was down-regulated in acute lymphoblastic leukemia. Moreover, hHK-1, in contrast to SP, was able to induce proliferation of human pre-B lymphocytes through a NK1 receptor-independent mechanism. These data suggest a role for hHK-1 in normal and pathological B lymphopoiesis, and open the door to a better understanding of the physiopathological mechanisms leading to lymphoproliferative malignancies.
AB - Tachykinins are a family of structurally related peptides, including substance P (SP), hemokinin-1 (HK-1), neurokinin A (NKA), and neurokinin B. SP and NKA have been shown to modulate hematopoiesis and rat/mouse HK-1 has been found to be involved in the survival and differentiation of mouse B-cells. This study was designed to assess the expression of tachykinins with a focus on human HK-1 (hHK-1) in human B lymphocytes and the role of these peptides in cell differentiation, apoptosis and proliferation. Expression of tachykinin and tachykinin receptor mRNA was determined quantitatively in human B lymphoproliferative malignancies and compared to normal B-cells. Expression of hHK-1 and NK1 receptor, but not SP, was detected in human B-lymphocytes, and was up-regulated in B-lymphocytes from chronic lymphocytic leukemia and non-Hodgkin's lymphoma, while it was down-regulated in acute lymphoblastic leukemia. Moreover, hHK-1, in contrast to SP, was able to induce proliferation of human pre-B lymphocytes through a NK1 receptor-independent mechanism. These data suggest a role for hHK-1 in normal and pathological B lymphopoiesis, and open the door to a better understanding of the physiopathological mechanisms leading to lymphoproliferative malignancies.
KW - B-cells
KW - Human hemokinin-1
KW - Proliferation
KW - Tachykinin receptors
UR - http://www.scopus.com/inward/record.url?scp=79955469191&partnerID=8YFLogxK
U2 - 10.1016/j.peptides.2011.02.012
DO - 10.1016/j.peptides.2011.02.012
M3 - Article
C2 - 21334411
AN - SCOPUS:79955469191
SN - 0196-9781
VL - 32
SP - 1027
EP - 1034
JO - Peptides
JF - Peptides
IS - 5
ER -