Fate Mapping via Ms4a3-Expression History Traces Monocyte-Derived Cells

Zhaoyuan Liu; Yaqi Gu; Svetoslav Chakarov; Camille Bleriot; Immanuel Kwok; Xin Chen; Amanda Shin; Weijie Huang; Regine J. Dress; Charles Antoine Dutertre; Andreas Schlitzer; Jinmiao Chen; Lai Guan Ng; Honglin Wang; Zhiduo Liu; Bing Su; Florent Ginhoux

doi:10.1016/j.cell.2019.08.009

Fate Mapping via Ms4a3-Expression History Traces Monocyte-Derived Cells

Zhaoyuan Liu, Yaqi Gu, Svetoslav Chakarov, Camille Bleriot, Immanuel Kwok, Xin Chen, Amanda Shin, Weijie Huang, Regine J. Dress, Charles Antoine Dutertre, Andreas Schlitzer, Jinmiao Chen, Lai Guan Ng, Honglin Wang, Zhiduo Liu, Bing Su, Florent Ginhoux

Research output: Contribution to journal › Article › peer-review

357 Citations (Scopus)

Abstract

Most tissue-resident macrophage (RTM) populations are seeded by waves of embryonic hematopoiesis and are self-maintained independently of a bone marrow contribution during adulthood. A proportion of RTMs, however, is constantly replaced by blood monocytes, and their functions compared to embryonic RTMs remain unclear. The kinetics and extent of the contribution of circulating monocytes to RTM replacement during homeostasis, inflammation, and disease are highly debated. Here, we identified Ms4a3 as a specific gene expressed by granulocyte-monocyte progenitors (GMPs) and subsequently generated Ms4a3^TdT reporter, Ms4a3^Cre, and Ms4a3^CreERT2 fate-mapping models. These models traced efficiently monocytes and granulocytes, but no lymphocytes or tissue dendritic cells. Using these models, we precisely quantified the contribution of monocytes to the RTM pool during homeostasis and inflammation. The unambiguous identification of monocyte-derived cells will permit future studies of their function under any condition.

Original language	English
Pages (from-to)	1509-1525.e19
Journal	Cell
Volume	178
Issue number	6
DOIs	https://doi.org/10.1016/j.cell.2019.08.009
Publication status	Published - 5 Sept 2019
Externally published	Yes

Keywords

GMP
MDP
Ms4a3
dendritic cell
fate mapping
granulocyte-monocyte progenitor
inflammation
monocyte
monocyte-dendritic cell progenitor
tissue-resident macrophage

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10.1016/j.cell.2019.08.009

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title = "Fate Mapping via Ms4a3-Expression History Traces Monocyte-Derived Cells",

abstract = "Most tissue-resident macrophage (RTM) populations are seeded by waves of embryonic hematopoiesis and are self-maintained independently of a bone marrow contribution during adulthood. A proportion of RTMs, however, is constantly replaced by blood monocytes, and their functions compared to embryonic RTMs remain unclear. The kinetics and extent of the contribution of circulating monocytes to RTM replacement during homeostasis, inflammation, and disease are highly debated. Here, we identified Ms4a3 as a specific gene expressed by granulocyte-monocyte progenitors (GMPs) and subsequently generated Ms4a3TdT reporter, Ms4a3Cre, and Ms4a3CreERT2 fate-mapping models. These models traced efficiently monocytes and granulocytes, but no lymphocytes or tissue dendritic cells. Using these models, we precisely quantified the contribution of monocytes to the RTM pool during homeostasis and inflammation. The unambiguous identification of monocyte-derived cells will permit future studies of their function under any condition.",

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author = "Zhaoyuan Liu and Yaqi Gu and Svetoslav Chakarov and Camille Bleriot and Immanuel Kwok and Xin Chen and Amanda Shin and Weijie Huang and Dress, {Regine J.} and Dutertre, {Charles Antoine} and Andreas Schlitzer and Jinmiao Chen and Ng, {Lai Guan} and Honglin Wang and Zhiduo Liu and Bing Su and Florent Ginhoux",

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year = "2019",

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doi = "10.1016/j.cell.2019.08.009",

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T1 - Fate Mapping via Ms4a3-Expression History Traces Monocyte-Derived Cells

AU - Liu, Zhaoyuan

AU - Gu, Yaqi

AU - Chakarov, Svetoslav

AU - Bleriot, Camille

AU - Kwok, Immanuel

AU - Chen, Xin

AU - Shin, Amanda

AU - Huang, Weijie

AU - Dress, Regine J.

AU - Dutertre, Charles Antoine

AU - Schlitzer, Andreas

AU - Chen, Jinmiao

AU - Ng, Lai Guan

AU - Wang, Honglin

AU - Liu, Zhiduo

AU - Su, Bing

AU - Ginhoux, Florent

PY - 2019/9/5

Y1 - 2019/9/5

N2 - Most tissue-resident macrophage (RTM) populations are seeded by waves of embryonic hematopoiesis and are self-maintained independently of a bone marrow contribution during adulthood. A proportion of RTMs, however, is constantly replaced by blood monocytes, and their functions compared to embryonic RTMs remain unclear. The kinetics and extent of the contribution of circulating monocytes to RTM replacement during homeostasis, inflammation, and disease are highly debated. Here, we identified Ms4a3 as a specific gene expressed by granulocyte-monocyte progenitors (GMPs) and subsequently generated Ms4a3TdT reporter, Ms4a3Cre, and Ms4a3CreERT2 fate-mapping models. These models traced efficiently monocytes and granulocytes, but no lymphocytes or tissue dendritic cells. Using these models, we precisely quantified the contribution of monocytes to the RTM pool during homeostasis and inflammation. The unambiguous identification of monocyte-derived cells will permit future studies of their function under any condition.

AB - Most tissue-resident macrophage (RTM) populations are seeded by waves of embryonic hematopoiesis and are self-maintained independently of a bone marrow contribution during adulthood. A proportion of RTMs, however, is constantly replaced by blood monocytes, and their functions compared to embryonic RTMs remain unclear. The kinetics and extent of the contribution of circulating monocytes to RTM replacement during homeostasis, inflammation, and disease are highly debated. Here, we identified Ms4a3 as a specific gene expressed by granulocyte-monocyte progenitors (GMPs) and subsequently generated Ms4a3TdT reporter, Ms4a3Cre, and Ms4a3CreERT2 fate-mapping models. These models traced efficiently monocytes and granulocytes, but no lymphocytes or tissue dendritic cells. Using these models, we precisely quantified the contribution of monocytes to the RTM pool during homeostasis and inflammation. The unambiguous identification of monocyte-derived cells will permit future studies of their function under any condition.

KW - GMP

KW - MDP

KW - Ms4a3

KW - dendritic cell

KW - fate mapping

KW - granulocyte-monocyte progenitor

KW - inflammation

KW - monocyte

KW - monocyte-dendritic cell progenitor

KW - tissue-resident macrophage

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DO - 10.1016/j.cell.2019.08.009

M3 - Article

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AN - SCOPUS:85071401707

SN - 0092-8674

VL - 178

SP - 1509-1525.e19

JO - Cell

JF - Cell

IS - 6

ER -

Fate Mapping via Ms4a3-Expression History Traces Monocyte-Derived Cells

Abstract

Keywords

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