Filter-adapted fluorescent in situ hybridization (FA-FISH) for filtration-enriched circulating tumor cells

Marianne Oulhen, Emma Pailler, Vincent Faugeroux, Françoise Farace

    Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

    2 Citations (Scopus)

    Abstract

    Circulating tumor cells (CTCs) may represent an easily accessible source of tumor material to assess genetic aberrations such as gene-rearrangements or gene-amplifications and screen cancer patients eligible for targeted therapies. As the number of CTCs is a critical parameter to identify such biomarkers, we developed fluorescent in situ hybridization (FISH) for CTCs enriched on filters (filter-adapted-FISH, FA-FISH). Here, we describe the FA-FISH protocol, the combination of immunofluorescent staining (DAPI/CD45) and FA-FISH techniques, as well as the semi-automated microscopy method that we developed to improve the feasibility and reliability of FISH analyses in filtration-enriched CTC.

    Original languageEnglish
    Title of host publicationMethods in Molecular Biology
    PublisherHumana Press Inc.
    Pages133-141
    Number of pages9
    DOIs
    Publication statusPublished - 1 Jan 2017

    Publication series

    NameMethods in Molecular Biology
    Volume1634
    ISSN (Print)1064-3745

    Keywords

    • Circulating tumor cells
    • FA-FISH
    • FISH
    • Filtration-enrichment
    • ISET

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