Abstract
The third edition of Flow Cytometry Guidelines provides the key aspects to consider when performing flow cytometry experiments and includes comprehensive sections describing phenotypes and functional assays of all major human and murine immune cell subsets. Notably, the Guidelines contain helpful tables highlighting phenotypes and key differences between human and murine cells. Another useful feature of this edition is the flow cytometry analysis of clinical samples with examples of flow cytometry applications in the context of autoimmune diseases, cancers as well as acute and chronic infectious diseases. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid. All sections are written and peer-reviewed by leading flow cytometry experts and immunologists, making this edition an essential and state-of-the-art handbook for basic and clinical researchers.
| Original language | English |
|---|---|
| Pages (from-to) | 2708-3145 |
| Number of pages | 438 |
| Journal | European Journal of Immunology |
| Volume | 51 |
| Issue number | 12 |
| DOIs | |
| Publication status | Published - 1 Dec 2021 |
| Externally published | Yes |
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In: European Journal of Immunology, Vol. 51, No. 12, 01.12.2021, p. 2708-3145.
Research output: Contribution to journal › Article › peer-review
TY - JOUR
T1 - Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition)
AU - Cossarizza, Andrea
AU - Chang, Hyun Dong
AU - Radbruch, Andreas
AU - Abrignani, Sergio
AU - Addo, Richard
AU - Akdis, Mübeccel
AU - Andrä, Immanuel
AU - Andreata, Francesco
AU - Annunziato, Francesco
AU - Arranz, Eduardo
AU - Bacher, Petra
AU - Bari, Sudipto
AU - Barnaba, Vincenzo
AU - Barros-Martins, Joana
AU - Baumjohann, Dirk
AU - Beccaria, Cristian G.
AU - Bernardo, David
AU - Boardman, Dominic A.
AU - Borger, Jessica
AU - Böttcher, Chotima
AU - Brockmann, Leonie
AU - Burns, Marie
AU - Busch, Dirk H.
AU - Cameron, Garth
AU - Cammarata, Ilenia
AU - Cassotta, Antonino
AU - Chang, Yinshui
AU - Chirdo, Fernando Gabriel
AU - Christakou, Eleni
AU - Čičin-Šain, Luka
AU - Cook, Laura
AU - Corbett, Alexandra J.
AU - Cornelis, Rebecca
AU - Cosmi, Lorenzo
AU - Davey, Martin S.
AU - De Biasi, Sara
AU - De Simone, Gabriele
AU - del Zotto, Genny
AU - Delacher, Michael
AU - Di Rosa, Francesca
AU - Santo, James Di
AU - Diefenbach, Andreas
AU - Dong, Jun
AU - Dörner, Thomas
AU - Dress, Regine J.
AU - Dutertre, Charles Antoine
AU - Eckle, Sidonia B.G.
AU - Eede, Pascale
AU - Evrard, Maximilien
AU - Falk, Christine S.
AU - Feuerer, Markus
AU - Fillatreau, Simon
AU - Fiz-Lopez, Aida
AU - Follo, Marie
AU - Foulds, Gemma A.
AU - Fröbel, Julia
AU - Gagliani, Nicola
AU - Galletti, Giovanni
AU - Gangaev, Anastasia
AU - Garbi, Natalio
AU - Garrote, José Antonio
AU - Geginat, Jens
AU - Gherardin, Nicholas A.
AU - Gibellini, Lara
AU - Ginhoux, Florent
AU - Godfrey, Dale I.
AU - Gruarin, Paola
AU - Haftmann, Claudia
AU - Hansmann, Leo
AU - Harpur, Christopher M.
AU - Hayday, Adrian C.
AU - Heine, Guido
AU - Hernández, Daniela Carolina
AU - Herrmann, Martin
AU - Hoelsken, Oliver
AU - Huang, Qing
AU - Huber, Samuel
AU - Huber, Johanna E.
AU - Huehn, Jochen
AU - Hundemer, Michael
AU - Hwang, William Y.K.
AU - Iannacone, Matteo
AU - Ivison, Sabine M.
AU - Jäck, Hans Martin
AU - Jani, Peter K.
AU - Keller, Baerbel
AU - Kessler, Nina
AU - Ketelaars, Steven
AU - Knop, Laura
AU - Knopf, Jasmin
AU - Koay, Hui Fern
AU - Kobow, Katja
AU - Kriegsmann, Katharina
AU - Kristyanto, H.
AU - Krueger, Andreas
AU - Kuehne, Jenny F.
AU - Kunze-Schumacher, Heike
AU - Kvistborg, Pia
AU - Kwok, Immanuel
AU - Latorre, Daniela
AU - Lenz, Daniel
AU - Levings, Megan K.
AU - Lino, Andreia C.
AU - Liotta, Francesco
AU - Long, Heather M.
AU - Lugli, Enrico
AU - MacDonald, Katherine N.
AU - Maggi, Laura
AU - Maini, Mala K.
AU - Mair, Florian
AU - Manta, Calin
AU - Manz, Rudolf Armin
AU - Mashreghi, Mir Farzin
AU - Mazzoni, Alessio
AU - McCluskey, James
AU - Mei, Henrik E.
AU - Melchers, Fritz
AU - Melzer, Susanne
AU - Mielenz, Dirk
AU - Monin, Leticia
AU - Moretta, Lorenzo
AU - Multhoff, Gabriele
AU - Muñoz, Luis Enrique
AU - Muñoz-Ruiz, Miguel
AU - Muscate, Franziska
AU - Natalini, Ambra
AU - Neumann, Katrin
AU - Ng, Lai Guan
AU - Niedobitek, Antonia
AU - Niemz, Jana
AU - Almeida, Larissa Nogueira
AU - Notarbartolo, Samuele
AU - Ostendorf, Lennard
AU - Pallett, Laura J.
AU - Patel, Amit A.
AU - Percin, Gulce Itir
AU - Peruzzi, Giovanna
AU - Pinti, Marcello
AU - Pockley, A. Graham
AU - Pracht, Katharina
AU - Prinz, Immo
AU - Pujol-Autonell, Irma
AU - Pulvirenti, Nadia
AU - Quatrini, Linda
AU - Quinn, Kylie M.
AU - Radbruch, Helena
AU - Rhys, Hefin
AU - Rodrigo, Maria B.
AU - Romagnani, Chiara
AU - Saggau, Carina
AU - Sakaguchi, Shimon
AU - Sallusto, Federica
AU - Sanderink, Lieke
AU - Sandrock, Inga
AU - Schauer, Christine
AU - Scheffold, Alexander
AU - Scherer, Hans U.
AU - Schiemann, Matthias
AU - Schildberg, Frank A.
AU - Schober, Kilian
AU - Schoen, Janina
AU - Schuh, Wolfgang
AU - Schüler, Thomas
AU - Schulz, Axel R.
AU - Schulz, Sebastian
AU - Schulze, Julia
AU - Simonetti, Sonia
AU - Singh, Jeeshan
AU - Sitnik, Katarzyna M.
AU - Stark, Regina
AU - Starossom, Sarah
AU - Stehle, Christina
AU - Szelinski, Franziska
AU - Tan, Leonard
AU - Tarnok, Attila
AU - Tornack, Julia
AU - Tree, Timothy I.M.
AU - van Beek, Jasper J.P.
AU - van de Veen, Willem
AU - van Gisbergen, Klaas
AU - Vasco, Chiara
AU - Verheyden, Nikita A.
AU - von Borstel, Anouk
AU - Ward-Hartstonge, Kirsten A.
AU - Warnatz, Klaus
AU - Waskow, Claudia
AU - Wiedemann, Annika
AU - Wilharm, Anneke
AU - Wing, James
AU - Wirz, Oliver
AU - Wittner, Jens
AU - Yang, Jennie H.M.
AU - Yang, Juhao
N1 - Publisher Copyright: © 2021 Wiley-VCH GmbH
PY - 2021/12/1
Y1 - 2021/12/1
N2 - The third edition of Flow Cytometry Guidelines provides the key aspects to consider when performing flow cytometry experiments and includes comprehensive sections describing phenotypes and functional assays of all major human and murine immune cell subsets. Notably, the Guidelines contain helpful tables highlighting phenotypes and key differences between human and murine cells. Another useful feature of this edition is the flow cytometry analysis of clinical samples with examples of flow cytometry applications in the context of autoimmune diseases, cancers as well as acute and chronic infectious diseases. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid. All sections are written and peer-reviewed by leading flow cytometry experts and immunologists, making this edition an essential and state-of-the-art handbook for basic and clinical researchers.
AB - The third edition of Flow Cytometry Guidelines provides the key aspects to consider when performing flow cytometry experiments and includes comprehensive sections describing phenotypes and functional assays of all major human and murine immune cell subsets. Notably, the Guidelines contain helpful tables highlighting phenotypes and key differences between human and murine cells. Another useful feature of this edition is the flow cytometry analysis of clinical samples with examples of flow cytometry applications in the context of autoimmune diseases, cancers as well as acute and chronic infectious diseases. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid. All sections are written and peer-reviewed by leading flow cytometry experts and immunologists, making this edition an essential and state-of-the-art handbook for basic and clinical researchers.
UR - http://www.scopus.com/inward/record.url?scp=85119620202&partnerID=8YFLogxK
U2 - 10.1002/eji.202170126
DO - 10.1002/eji.202170126
M3 - Article
C2 - 34910301
AN - SCOPUS:85119620202
SN - 0014-2980
VL - 51
SP - 2708
EP - 3145
JO - European Journal of Immunology
JF - European Journal of Immunology
IS - 12
ER -