High-performance thin-layer chromatography with a derivatization procedure, a suitable method for the identification and the quantitation of busulfan in various pharmaceutical products

Jérôme Bouligand; Angelo Paci; Lionel Mercier; Gilles Vassal; Philippe Bourget

doi:10.1016/S0731-7085(03)00630-7

High-performance thin-layer chromatography with a derivatization procedure, a suitable method for the identification and the quantitation of busulfan in various pharmaceutical products

Jérôme Bouligand, Angelo Paci, Lionel Mercier, Gilles Vassal, Philippe Bourget

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

Busulfan is an alkylating agent widely used in combination chemotherapy regimens followed by allogeneic or autologous hematopoietic stem cell transplantation (HSCT). We present a rapid method for assaying busulfan in pharmaceutical preparations using high-performance thin-layer chromatography (HPTLC) and derivatization with 4-nitrobenzylpyridine. The method is accurate and precise and allows quantitation of busulfan in aqueous solutions from 100 to 500μg/ml. It is suitable for identification, quantitation and stability studies of busulfan in pharmaceutical products, i.e. capsules or infusion bags prepared in a hospital pharmacy.

Original language	English
Pages (from-to)	525-530
Number of pages	6
Journal	Journal of Pharmaceutical and Biomedical Analysis
Volume	34
Issue number	3
DOIs	https://doi.org/10.1016/S0731-7085(03)00630-7
Publication status	Published - 18 Feb 2004

Keywords

4-Nitrobenzylpyridine
Busulfan
Cyclophosphamide
Derivatization
HPTLC
Quality control

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10.1016/S0731-7085(03)00630-7

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Bouligand, J., Paci, A., Mercier, L., Vassal, G., & Bourget, P. (2004). High-performance thin-layer chromatography with a derivatization procedure, a suitable method for the identification and the quantitation of busulfan in various pharmaceutical products. Journal of Pharmaceutical and Biomedical Analysis, 34(3), 525-530. https://doi.org/10.1016/S0731-7085(03)00630-7

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Bouligand, J, Paci, A, Mercier, L, Vassal, G & Bourget, P 2004, 'High-performance thin-layer chromatography with a derivatization procedure, a suitable method for the identification and the quantitation of busulfan in various pharmaceutical products', Journal of Pharmaceutical and Biomedical Analysis, vol. 34, no. 3, pp. 525-530. https://doi.org/10.1016/S0731-7085(03)00630-7

High-performance thin-layer chromatography with a derivatization procedure, a suitable method for the identification and the quantitation of busulfan in various pharmaceutical products. / Bouligand, Jérôme; Paci, Angelo; Mercier, Lionel et al.
In: Journal of Pharmaceutical and Biomedical Analysis, Vol. 34, No. 3, 18.02.2004, p. 525-530.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - High-performance thin-layer chromatography with a derivatization procedure, a suitable method for the identification and the quantitation of busulfan in various pharmaceutical products

AU - Bouligand, Jérôme

AU - Paci, Angelo

AU - Mercier, Lionel

AU - Vassal, Gilles

AU - Bourget, Philippe

PY - 2004/2/18

Y1 - 2004/2/18

N2 - Busulfan is an alkylating agent widely used in combination chemotherapy regimens followed by allogeneic or autologous hematopoietic stem cell transplantation (HSCT). We present a rapid method for assaying busulfan in pharmaceutical preparations using high-performance thin-layer chromatography (HPTLC) and derivatization with 4-nitrobenzylpyridine. The method is accurate and precise and allows quantitation of busulfan in aqueous solutions from 100 to 500μg/ml. It is suitable for identification, quantitation and stability studies of busulfan in pharmaceutical products, i.e. capsules or infusion bags prepared in a hospital pharmacy.

AB - Busulfan is an alkylating agent widely used in combination chemotherapy regimens followed by allogeneic or autologous hematopoietic stem cell transplantation (HSCT). We present a rapid method for assaying busulfan in pharmaceutical preparations using high-performance thin-layer chromatography (HPTLC) and derivatization with 4-nitrobenzylpyridine. The method is accurate and precise and allows quantitation of busulfan in aqueous solutions from 100 to 500μg/ml. It is suitable for identification, quantitation and stability studies of busulfan in pharmaceutical products, i.e. capsules or infusion bags prepared in a hospital pharmacy.

KW - 4-Nitrobenzylpyridine

KW - Busulfan

KW - Cyclophosphamide

KW - Derivatization

KW - HPTLC

KW - Quality control

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DO - 10.1016/S0731-7085(03)00630-7

M3 - Article

C2 - 15127808

AN - SCOPUS:0842346337

SN - 0731-7085

VL - 34

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JO - Journal of Pharmaceutical and Biomedical Analysis

JF - Journal of Pharmaceutical and Biomedical Analysis

IS - 3

ER -

High-performance thin-layer chromatography with a derivatization procedure, a suitable method for the identification and the quantitation of busulfan in various pharmaceutical products

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Keywords

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