High throughput/high content capture and typing of rare cells for cancer diagnosis and prognosis

L. Saias, A. E. Saliba, J. Autebert, J. Y. Pierga, P. Vielh, F. Farace, L. Malaquin, J. L. Viovy

    Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

    1 Citation (Scopus)

    Abstract

    We present a new integrated microfluidic system for the high throughput sorting of rare cells (few tens of cells per millilitre of sample) and their in-situ analysis by bright field and fluorescence microscopy at high magnification. The sample flows through an array of magnetic columns coated with antibodies against surface antigens of the target cells.. We present here a characterization of the system's physical principles and performances, and developments towards the sorting of epithelial tumour cells from the blood of breast cancer patients.

    Original languageEnglish
    Title of host publicationProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
    PublisherChemical and Biological Microsystems Society
    Pages660-662
    Number of pages3
    ISBN (Print)9780979806421
    Publication statusPublished - 1 Jan 2009
    Event13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 - Jeju, Korea, Republic of
    Duration: 1 Nov 20095 Nov 2009

    Publication series

    NameProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences

    Conference

    Conference13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009
    Country/TerritoryKorea, Republic of
    CityJeju
    Period1/11/095/11/09

    Keywords

    • Cancer diagnosis
    • Cell sorting
    • High throughput
    • Magnetic particles

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