Imaging green fluorescent protein fusions in living fission yeast cells

P. T. Tran; Anne Paoletti; Fred Chang

doi:10.1016/j.ymeth.2003.11.017

Imaging green fluorescent protein fusions in living fission yeast cells

P. T. Tran, Anne Paoletti, Fred Chang

Research output: Contribution to journal › Article › peer-review

80 Citations (Scopus)

Abstract

The use of green fluorescent protein (GFP) fusions as biosensors for examining protein localization and dynamics has revolutionized cell biology. Here, we describe the methods developed for imaging of GFP-fusions in the fission yeast Schizosaccharomyces pombe using fluorescence microscopy, with a focus on the use of time-lapse imaging to analyze the dynamics of microtubules. We discuss the considerations in fluorescence microscopy, cell preparation, data acquisition, and image analysis appropriate for analysis of living cells.

Original language	English
Pages (from-to)	220-225
Number of pages	6
Journal	Methods
Volume	33
Issue number	3
DOIs	https://doi.org/10.1016/j.ymeth.2003.11.017
Publication status	Published - 1 Jul 2004
Externally published	Yes

Keywords

Confocal
Digital imaging
Fluorescence
GFP
Microscope
Microtubules
Time-lapse

Access to Document

10.1016/j.ymeth.2003.11.017

Cite this

@article{380a616659ac4981b5a46c0480aac2ad,

title = "Imaging green fluorescent protein fusions in living fission yeast cells",

abstract = "The use of green fluorescent protein (GFP) fusions as biosensors for examining protein localization and dynamics has revolutionized cell biology. Here, we describe the methods developed for imaging of GFP-fusions in the fission yeast Schizosaccharomyces pombe using fluorescence microscopy, with a focus on the use of time-lapse imaging to analyze the dynamics of microtubules. We discuss the considerations in fluorescence microscopy, cell preparation, data acquisition, and image analysis appropriate for analysis of living cells.",

keywords = "Confocal, Digital imaging, Fluorescence, GFP, Microscope, Microtubules, Time-lapse",

author = "Tran, {P. T.} and Anne Paoletti and Fred Chang",

year = "2004",

month = jul,

day = "1",

doi = "10.1016/j.ymeth.2003.11.017",

language = "English",

volume = "33",

pages = "220--225",

journal = "Methods",

issn = "1046-2023",

number = "3",

}

TY - JOUR

T1 - Imaging green fluorescent protein fusions in living fission yeast cells

AU - Tran, P. T.

AU - Paoletti, Anne

AU - Chang, Fred

PY - 2004/7/1

Y1 - 2004/7/1

N2 - The use of green fluorescent protein (GFP) fusions as biosensors for examining protein localization and dynamics has revolutionized cell biology. Here, we describe the methods developed for imaging of GFP-fusions in the fission yeast Schizosaccharomyces pombe using fluorescence microscopy, with a focus on the use of time-lapse imaging to analyze the dynamics of microtubules. We discuss the considerations in fluorescence microscopy, cell preparation, data acquisition, and image analysis appropriate for analysis of living cells.

AB - The use of green fluorescent protein (GFP) fusions as biosensors for examining protein localization and dynamics has revolutionized cell biology. Here, we describe the methods developed for imaging of GFP-fusions in the fission yeast Schizosaccharomyces pombe using fluorescence microscopy, with a focus on the use of time-lapse imaging to analyze the dynamics of microtubules. We discuss the considerations in fluorescence microscopy, cell preparation, data acquisition, and image analysis appropriate for analysis of living cells.

KW - Confocal

KW - Digital imaging

KW - Fluorescence

KW - GFP

KW - Microscope

KW - Microtubules

KW - Time-lapse

UR - http://www.scopus.com/inward/record.url?scp=2442553594&partnerID=8YFLogxK

U2 - 10.1016/j.ymeth.2003.11.017

DO - 10.1016/j.ymeth.2003.11.017

M3 - Article

C2 - 15157889

AN - SCOPUS:2442553594

SN - 1046-2023

VL - 33

SP - 220

EP - 225

JO - Methods

JF - Methods

IS - 3

ER -

Imaging green fluorescent protein fusions in living fission yeast cells

Abstract

Keywords

Access to Document

Other files and links

Cite this