In vitro characterization of gap junctional intercellular communication by gap-FRAP technique

M. Abbaci; J. R. Stines; M. Barberi-Heyob; W. Blondel; D. Dumas; F. Guillemin; J. Didelon

In vitro characterization of gap junctional intercellular communication by gap-FRAP technique

M. Abbaci, J. R. Stines, M. Barberi-Heyob, W. Blondel, D. Dumas, F. Guillemin, J. Didelon

Research output: Contribution to journal › Conference article › peer-review

2 Citations (Scopus)

Abstract

Gap junctional intercellular communication (GJIC) has been shown to be involved in the carcinogenesis process. Gap-FRAP (Fluorescence Recovery After Photobleaching) technique could be used to estimate gap junctions functionality and their potential involvement for distinguish normal and cancer cells. In this study, the gap-FRAP technique was used to analyse functional gap-junction-mediated communication for cell lines with different GJIC status. Gap-FRAP data and connexin 43 protein expression decreased for FaDu cancer cell line, in contrast to fibroblast and KB positives cell lines. To check the involvement and functionality of gap junctions in the restitution of the fluorescence after photobleaching, we used a gap junction channel inhibition assay with 18 α-glycyrrhetinic acid. Our results indicate that the degree of gap junctional intercellular communication could be estimated by this technique in vitro.

Original language	English
Article number	585909
Pages (from-to)	1-8
Number of pages	8
Journal	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	5859
Publication status	Published - 19 Dec 2005
Externally published	Yes
Event	Photon Migration and Diffuse-Light Imaging II - Munich, Germany Duration: 12 Jun 2005 → 16 Jun 2005

Keywords

Connexin 43
Gap junction
Gap-FRAP

Cite this

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title = "In vitro characterization of gap junctional intercellular communication by gap-FRAP technique",

abstract = "Gap junctional intercellular communication (GJIC) has been shown to be involved in the carcinogenesis process. Gap-FRAP (Fluorescence Recovery After Photobleaching) technique could be used to estimate gap junctions functionality and their potential involvement for distinguish normal and cancer cells. In this study, the gap-FRAP technique was used to analyse functional gap-junction-mediated communication for cell lines with different GJIC status. Gap-FRAP data and connexin 43 protein expression decreased for FaDu cancer cell line, in contrast to fibroblast and KB positives cell lines. To check the involvement and functionality of gap junctions in the restitution of the fluorescence after photobleaching, we used a gap junction channel inhibition assay with 18 α-glycyrrhetinic acid. Our results indicate that the degree of gap junctional intercellular communication could be estimated by this technique in vitro.",

keywords = "Connexin 43, Gap junction, Gap-FRAP",

author = "M. Abbaci and Stines, {J. R.} and M. Barberi-Heyob and W. Blondel and D. Dumas and F. Guillemin and J. Didelon",

year = "2005",

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language = "English",

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pages = "1--8",

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TY - JOUR

T1 - In vitro characterization of gap junctional intercellular communication by gap-FRAP technique

AU - Abbaci, M.

AU - Stines, J. R.

AU - Barberi-Heyob, M.

AU - Blondel, W.

AU - Dumas, D.

AU - Guillemin, F.

AU - Didelon, J.

PY - 2005/12/19

Y1 - 2005/12/19

N2 - Gap junctional intercellular communication (GJIC) has been shown to be involved in the carcinogenesis process. Gap-FRAP (Fluorescence Recovery After Photobleaching) technique could be used to estimate gap junctions functionality and their potential involvement for distinguish normal and cancer cells. In this study, the gap-FRAP technique was used to analyse functional gap-junction-mediated communication for cell lines with different GJIC status. Gap-FRAP data and connexin 43 protein expression decreased for FaDu cancer cell line, in contrast to fibroblast and KB positives cell lines. To check the involvement and functionality of gap junctions in the restitution of the fluorescence after photobleaching, we used a gap junction channel inhibition assay with 18 α-glycyrrhetinic acid. Our results indicate that the degree of gap junctional intercellular communication could be estimated by this technique in vitro.

AB - Gap junctional intercellular communication (GJIC) has been shown to be involved in the carcinogenesis process. Gap-FRAP (Fluorescence Recovery After Photobleaching) technique could be used to estimate gap junctions functionality and their potential involvement for distinguish normal and cancer cells. In this study, the gap-FRAP technique was used to analyse functional gap-junction-mediated communication for cell lines with different GJIC status. Gap-FRAP data and connexin 43 protein expression decreased for FaDu cancer cell line, in contrast to fibroblast and KB positives cell lines. To check the involvement and functionality of gap junctions in the restitution of the fluorescence after photobleaching, we used a gap junction channel inhibition assay with 18 α-glycyrrhetinic acid. Our results indicate that the degree of gap junctional intercellular communication could be estimated by this technique in vitro.

KW - Connexin 43

KW - Gap junction

KW - Gap-FRAP

UR - http://www.scopus.com/inward/record.url?scp=28844493125&partnerID=8YFLogxK

M3 - Conference article

AN - SCOPUS:28844493125

SN - 1605-7422

VL - 5859

SP - 1

EP - 8

JO - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

JF - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

M1 - 585909

T2 - Photon Migration and Diffuse-Light Imaging II

Y2 - 12 June 2005 through 16 June 2005

ER -

In vitro characterization of gap junctional intercellular communication by gap-FRAP technique

Abstract

Keywords

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