Landscape of mast cell populations across organs in mice and humans

Marie Tauber; Lilian Basso; Jeremy Martin; Luciana Bostan; Marlene Magalhaes Pinto; Guilhem R. Thierry; Raïssa Houmadi; Nadine Serhan; Alexia Loste; Camille Blériot; Jasper B.J. Kamphuis; Mirjana Grujic; Lena Kjellén; Gunnar Pejler; Carle Paul; Xinzhong Dong; Stephen J. Galli; Laurent L. Reber; Florent Ginhoux; Marc Bajenoff; Rebecca Gentek; Nicolas Gaudenzio

doi:10.1084/jem.20230570

Landscape of mast cell populations across organs in mice and humans

Marie Tauber, Lilian Basso, Jeremy Martin, Luciana Bostan, Marlene Magalhaes Pinto, Guilhem R. Thierry, Raïssa Houmadi, Nadine Serhan, Alexia Loste, Camille Blériot, Jasper B.J. Kamphuis, Mirjana Grujic, Lena Kjellén, Gunnar Pejler, Carle Paul, Xinzhong Dong, Stephen J. Galli, Laurent L. Reber, Florent Ginhoux, Marc BajenoffRebecca Gentek, Nicolas Gaudenzio

Research output: Contribution to journal › Article › peer-review

48 Citations (Scopus)

Abstract

Mast cells (MCs) are tissue-resident immune cells that exhibit homeostatic and neuron-associated functions. Here, we combined whole-tissue imaging and single-cell RNA sequencing datasets to generate a pan-organ analysis of MCs in mice and humans at steady state. In mice, we identify two mutually exclusive MC populations, MrgprB2⁺ connective tissue–type MCs and MrgprB2^neg mucosal-type MCs, with specific transcriptomic core signatures. While MrgprB2⁺ MCs develop in utero independently of the bone marrow, MrgprB2^neg MCs develop after birth and are renewed by bone marrow progenitors. In humans, we unbiasedly identify seven MC subsets (MC1–7) distributed across 12 organs with different transcriptomic core signatures. MC1 are preferentially enriched in the bladder, MC2 in the lungs, and MC4, MC6, and MC7 in the skin. Conversely, MC3 and MC5 are shared by most organs but not skin. This comprehensive analysis offers valuable insights into the natural diversity of MC subtypes in both mice and humans.

Original language	English
Article number	e20230570
Journal	Journal of Experimental Medicine
Volume	220
Issue number	10
DOIs	https://doi.org/10.1084/jem.20230570
Publication status	Published - 2 Oct 2023

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10.1084/jem.20230570

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Tauber, M., Basso, L., Martin, J., Bostan, L., Pinto, M. M., Thierry, G. R., Houmadi, R., Serhan, N., Loste, A., Blériot, C., Kamphuis, J. B. J., Grujic, M., Kjellén, L., Pejler, G., Paul, C., Dong, X., Galli, S. J., Reber, L. L., Ginhoux, F., ... Gaudenzio, N. (2023). Landscape of mast cell populations across organs in mice and humans. Journal of Experimental Medicine, 220(10), Article e20230570. https://doi.org/10.1084/jem.20230570

@article{01a65ad386b6470ab95dd3205a494a13,

title = "Landscape of mast cell populations across organs in mice and humans",

abstract = "Mast cells (MCs) are tissue-resident immune cells that exhibit homeostatic and neuron-associated functions. Here, we combined whole-tissue imaging and single-cell RNA sequencing datasets to generate a pan-organ analysis of MCs in mice and humans at steady state. In mice, we identify two mutually exclusive MC populations, MrgprB2+ connective tissue–type MCs and MrgprB2neg mucosal-type MCs, with specific transcriptomic core signatures. While MrgprB2+ MCs develop in utero independently of the bone marrow, MrgprB2neg MCs develop after birth and are renewed by bone marrow progenitors. In humans, we unbiasedly identify seven MC subsets (MC1–7) distributed across 12 organs with different transcriptomic core signatures. MC1 are preferentially enriched in the bladder, MC2 in the lungs, and MC4, MC6, and MC7 in the skin. Conversely, MC3 and MC5 are shared by most organs but not skin. This comprehensive analysis offers valuable insights into the natural diversity of MC subtypes in both mice and humans.",

author = "Marie Tauber and Lilian Basso and Jeremy Martin and Luciana Bostan and Pinto, {Marlene Magalhaes} and Thierry, {Guilhem R.} and Ra{\"i}ssa Houmadi and Nadine Serhan and Alexia Loste and Camille Bl{\'e}riot and Kamphuis, {Jasper B.J.} and Mirjana Grujic and Lena Kjell{\'e}n and Gunnar Pejler and Carle Paul and Xinzhong Dong and Galli, {Stephen J.} and Reber, {Laurent L.} and Florent Ginhoux and Marc Bajenoff and Rebecca Gentek and Nicolas Gaudenzio",

note = "Publisher Copyright: {\textcopyright} 2023 Tauber et al.",

year = "2023",

month = oct,

day = "2",

doi = "10.1084/jem.20230570",

language = "English",

volume = "220",

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Tauber, M, Basso, L, Martin, J, Bostan, L, Pinto, MM, Thierry, GR, Houmadi, R, Serhan, N, Loste, A, Blériot, C, Kamphuis, JBJ, Grujic, M, Kjellén, L, Pejler, G, Paul, C, Dong, X, Galli, SJ, Reber, LL, Ginhoux, F, Bajenoff, M, Gentek, R & Gaudenzio, N 2023, 'Landscape of mast cell populations across organs in mice and humans', Journal of Experimental Medicine, vol. 220, no. 10, e20230570. https://doi.org/10.1084/jem.20230570

TY - JOUR

T1 - Landscape of mast cell populations across organs in mice and humans

AU - Tauber, Marie

AU - Basso, Lilian

AU - Martin, Jeremy

AU - Bostan, Luciana

AU - Pinto, Marlene Magalhaes

AU - Thierry, Guilhem R.

AU - Houmadi, Raïssa

AU - Serhan, Nadine

AU - Loste, Alexia

AU - Blériot, Camille

AU - Kamphuis, Jasper B.J.

AU - Grujic, Mirjana

AU - Kjellén, Lena

AU - Pejler, Gunnar

AU - Paul, Carle

AU - Dong, Xinzhong

AU - Galli, Stephen J.

AU - Reber, Laurent L.

AU - Ginhoux, Florent

AU - Bajenoff, Marc

AU - Gentek, Rebecca

AU - Gaudenzio, Nicolas

PY - 2023/10/2

Y1 - 2023/10/2

N2 - Mast cells (MCs) are tissue-resident immune cells that exhibit homeostatic and neuron-associated functions. Here, we combined whole-tissue imaging and single-cell RNA sequencing datasets to generate a pan-organ analysis of MCs in mice and humans at steady state. In mice, we identify two mutually exclusive MC populations, MrgprB2+ connective tissue–type MCs and MrgprB2neg mucosal-type MCs, with specific transcriptomic core signatures. While MrgprB2+ MCs develop in utero independently of the bone marrow, MrgprB2neg MCs develop after birth and are renewed by bone marrow progenitors. In humans, we unbiasedly identify seven MC subsets (MC1–7) distributed across 12 organs with different transcriptomic core signatures. MC1 are preferentially enriched in the bladder, MC2 in the lungs, and MC4, MC6, and MC7 in the skin. Conversely, MC3 and MC5 are shared by most organs but not skin. This comprehensive analysis offers valuable insights into the natural diversity of MC subtypes in both mice and humans.

AB - Mast cells (MCs) are tissue-resident immune cells that exhibit homeostatic and neuron-associated functions. Here, we combined whole-tissue imaging and single-cell RNA sequencing datasets to generate a pan-organ analysis of MCs in mice and humans at steady state. In mice, we identify two mutually exclusive MC populations, MrgprB2+ connective tissue–type MCs and MrgprB2neg mucosal-type MCs, with specific transcriptomic core signatures. While MrgprB2+ MCs develop in utero independently of the bone marrow, MrgprB2neg MCs develop after birth and are renewed by bone marrow progenitors. In humans, we unbiasedly identify seven MC subsets (MC1–7) distributed across 12 organs with different transcriptomic core signatures. MC1 are preferentially enriched in the bladder, MC2 in the lungs, and MC4, MC6, and MC7 in the skin. Conversely, MC3 and MC5 are shared by most organs but not skin. This comprehensive analysis offers valuable insights into the natural diversity of MC subtypes in both mice and humans.

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U2 - 10.1084/jem.20230570

DO - 10.1084/jem.20230570

M3 - Article

C2 - 37462672

AN - SCOPUS:85165521127

SN - 0022-1007

VL - 220

JO - Journal of Experimental Medicine

JF - Journal of Experimental Medicine

IS - 10

M1 - e20230570

ER -

Landscape of mast cell populations across organs in mice and humans

Abstract

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