Molecular cloning and characterization of the mouse Kin17 gene coding for a Zn-finger protein that preferentially recognizes bent DNA

Agnès Tissier, Patricia Kannouche, Philippe Mauffrey, Isabelle Allemand, Gérard Frelat, Raymond Devoret, Jaime F. Angulo

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

We report the isolation of the mouse Kin17 gene, located on chromosome 2, coding a nuclear Zn-finger protein that has a 39-residue region homologous to Escherichia coli RecA protein and that is recognized by anti- RecA antibodies. Kin17 protein preferentially binds to curved DNA in vitro and in vivo, suggesting a role in illegitimate recombination and in regulation of gene expression. We have shown that the Kin17 gene is about 8 kb in length and displays three exons and two introns. The 5' flanking region lacks a canonical TATAA box but presents several putative regulatory domains. A major transcription initiation site is located 322 nucleotides upstream of the translation start site. The 1.7-kb transcript of the Kin17 gene is weakly and ubiquitously expressed in murine tissues and cell lines as determined by Northern analysis. The cross-hybridization of Kin17 cDNA with the genomic DNA of other species in Southern analysis indicates the conservation of the gene among mammals and suggests that the Kin17 gene plays a conserved role in DNA metabolism.

Original languageEnglish
Pages (from-to)238-242
Number of pages5
JournalGenomics
Volume38
Issue number2
DOIs
Publication statusPublished - 1 Dec 1996
Externally publishedYes

Cite this