TY - JOUR
T1 - Molecular events involved in ochratoxin A induced mitochondrial pathway of apoptosis, modulation by Bcl-2 family members
AU - Bouaziz, Chayma
AU - Sharaf el dein, Ossama
AU - Martel, Cécile
AU - Golli, Emna El
AU - Abid-Essefi, Salwa
AU - Brenner, Catherine
AU - Lemaire, Christophe
AU - Bacha, Hassen
PY - 2011/11/1
Y1 - 2011/11/1
N2 - In this study, we looked for the role of the mitochondrion in the cytotoxicity of ochratoxin A (OTA), which is one of the most abundant food-contaminating mycotoxins in the world. In different human carcinoma cell lines, OTA triggered a mitochondria-dependent apoptotic process, which is characterized by opening of the mitochondrial permeability transition pore (PTPC), loss of mitochondrial transmembrane potential (ΔΨ m), increase in O 2[chemp] - production, mitochondrial relocalization of Bax, release of cytochrome c, and caspase activation. However, studies performed on purified organelles suggested that OTA does not directly target the mitochondrion. In addition, we showed that mitochondrial alterations induced by this mycotoxin are favored by the proapoptotic protein Bax, but not Bak. These alterations are prevented by the antiapoptotic proteins, Bcl-2 and to a lesser degree by Bcl-X L. Taken together, these data indicate that although mitochondria, PTPC members and proteins of Bcl-2 family play a pivotal role in OTA-induced apoptosis, they do not constitute real targets to overcome its toxicity.
AB - In this study, we looked for the role of the mitochondrion in the cytotoxicity of ochratoxin A (OTA), which is one of the most abundant food-contaminating mycotoxins in the world. In different human carcinoma cell lines, OTA triggered a mitochondria-dependent apoptotic process, which is characterized by opening of the mitochondrial permeability transition pore (PTPC), loss of mitochondrial transmembrane potential (ΔΨ m), increase in O 2[chemp] - production, mitochondrial relocalization of Bax, release of cytochrome c, and caspase activation. However, studies performed on purified organelles suggested that OTA does not directly target the mitochondrion. In addition, we showed that mitochondrial alterations induced by this mycotoxin are favored by the proapoptotic protein Bax, but not Bak. These alterations are prevented by the antiapoptotic proteins, Bcl-2 and to a lesser degree by Bcl-X L. Taken together, these data indicate that although mitochondria, PTPC members and proteins of Bcl-2 family play a pivotal role in OTA-induced apoptosis, they do not constitute real targets to overcome its toxicity.
KW - Apoptosis
KW - Bcl-2
KW - Mitochondria
KW - Ochratoxin A
KW - PTPC
KW - ROS
UR - http://www.scopus.com/inward/record.url?scp=80355141988&partnerID=8YFLogxK
U2 - 10.1002/tox.20581
DO - 10.1002/tox.20581
M3 - Article
C2 - 20549612
AN - SCOPUS:80355141988
SN - 1520-4081
VL - 26
SP - 579
EP - 590
JO - Environmental Toxicology
JF - Environmental Toxicology
IS - 6
ER -