TY - JOUR
T1 - Molecular genetic studies of chromosome 11 and chromosome 22q DNA sequences in pediatric medulloblastomas
AU - Lescop, Servane
AU - Lellouch-Tubiana, Arielle
AU - Vassal, Gilles
AU - Besnard-Guerin, Corinne
N1 - Funding Information:
This work was supported by the Association pour la Recherche contre le Cancer (ARC, Villejuif ) and INSERM (Institut National de la Santé et de la Recherche Médicale.). We thank the neurosurgeons from the Neckers-Enfants Malades Hospital and their staff for their help in providing tumor samples. We thank Dr. Feunteun and his staff, Dr. Kalifa, Dr. Brugières, Dr. Anne Abel and Dr. Agnes Chompret from the department of pediatric oncology from the Institute Gustave Roussy for their support in this work.
PY - 1999/1/1
Y1 - 1999/1/1
N2 - Medulloblastomas are primitive neuroectodermal tumors (PNETs) of the cerebellum with poorly understood pathogenesis. Previous molecular studies suggested a role for loci on chromosome 11 in the development of medulloblastomas-PNETs. In order to identify the frequency of loss and eventually the extent of allelic loss on chromosome 11, we have examined 23 pediatric medulloblastomas for loss of heterozygosity (LOH) with 16 polymorphic microsatellites. Our data reveal that LOH on 11p or 11q occurs rarely (13%) suggesting the unlikely involvement of chromosome 11 in most cases of medulloblastomas. The same frequency of LOH in medulloblastomas was detected using 8 microsatellites on 22q. Alterations of microsatellite length were found in only 4/594 PCR analyses using 28 markers located on chromosomes 2, 9, 11, 18, and 22, demonstrating that genomic instability is uncommon in medulloblastomas.
AB - Medulloblastomas are primitive neuroectodermal tumors (PNETs) of the cerebellum with poorly understood pathogenesis. Previous molecular studies suggested a role for loci on chromosome 11 in the development of medulloblastomas-PNETs. In order to identify the frequency of loss and eventually the extent of allelic loss on chromosome 11, we have examined 23 pediatric medulloblastomas for loss of heterozygosity (LOH) with 16 polymorphic microsatellites. Our data reveal that LOH on 11p or 11q occurs rarely (13%) suggesting the unlikely involvement of chromosome 11 in most cases of medulloblastomas. The same frequency of LOH in medulloblastomas was detected using 8 microsatellites on 22q. Alterations of microsatellite length were found in only 4/594 PCR analyses using 28 markers located on chromosomes 2, 9, 11, 18, and 22, demonstrating that genomic instability is uncommon in medulloblastomas.
KW - Chromosomes 11 and 22
KW - Instability
KW - Loss of heterozygosity
KW - Medulloblastoma
UR - http://www.scopus.com/inward/record.url?scp=0032761061&partnerID=8YFLogxK
U2 - 10.1023/A:1006387518100
DO - 10.1023/A:1006387518100
M3 - Article
C2 - 10619495
AN - SCOPUS:0032761061
SN - 0167-594X
VL - 44
SP - 119
EP - 127
JO - Journal of Neuro-Oncology
JF - Journal of Neuro-Oncology
IS - 2
ER -