Na+/I- symporter and Pendred syndrome gene and protein expressions in human extra-thyroidal tissues

L. Lacroix, C. Mian, B. Caillou, M. Talbot, S. Filetti, M. Schlumberger, J. M. Bidart

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    Abstract

    Objective: The expression of two recently identified iodide transporters, namely the sodium/iodide symporter (NIS) and pendrin, the product of the gene responsible for the Pendred syndrome (PDS), was studied in a series of various extra-thyroidal human tissues, and especially in those known to concentrate iodide. Methods: To this end, we used real-time kinetic quantitative PCR to detect NIS and PDS transcripts and immunohistochemistry for the analysis of their protein products. Results: NIS gene and protein expression was detected in most tissues known to concentrate iodine, and particularly in salivary glands and stomach. In contrast, PDS gene expression was restricted to a few tissues, such as kidney and Sertoli cells. Interestingly, in kidney, pendrin immunostaining was detected at the apical pole of epithelial cells of the thick ascending limb of the Henle's loop and of the distal convoluted tubule. Conclusion: This study provides new insights on the localization and expression of two genes involved in iodide transport and emphasizes the interest of combining real-time quantitative PCR and immunohistochemistry for the comparison of gene and protein expression in tissues.

    Original languageEnglish
    Pages (from-to)297-302
    Number of pages6
    JournalEuropean Journal of Endocrinology
    Volume144
    Issue number3
    DOIs
    Publication statusPublished - 1 Jan 2001

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