TY - JOUR
T1 - New hemodynamic approach to angiogenesis
T2 - Color and pulsed Doppler ultrasonography
AU - Lassau, Nathalie
AU - Paturel-Asselin, Carinne
AU - Guinebretiere, Jean Marc
AU - Leclère, Jerome
AU - Koscielny, Serge
AU - Roche, Alain
AU - Chouaib, Salem
AU - Peronneau, Pierre
PY - 1999/1/1
Y1 - 1999/1/1
N2 - RATIONALE AND OBJECTIVES. To determine the capacity of color and spectral Doppler ultrasonography (US) to quantify angiogenesis in vivo and to characterize low-resistance intratumor blood flow. METHODS. Thirty-two tumors, xenografted into mice, were studied with Doppler US. The number of intratumor vessels visualized with color Doppler US was compared with the density of microvessels and the number of vessels > 100 μm determined by histologic examination. The resistance index and the peak systolic velocities were evaluated. RESULTS. The number of intratumor vessels visualized by color Doppler US was correlated with the number of vessels >100 μm (P < 0.001) determined histologically. When vessel density was >30, intratumor vessels were always detected by color Doppler US. The resistance index and peak systolic velocities were significantly lower in intratumor than in peritumor vessels. CONCLUSIONS. Color Doppler US evaluated tumor angiogenesis accurately. Spectral analysis confirmed the low resistance of intratumor blood flow.
AB - RATIONALE AND OBJECTIVES. To determine the capacity of color and spectral Doppler ultrasonography (US) to quantify angiogenesis in vivo and to characterize low-resistance intratumor blood flow. METHODS. Thirty-two tumors, xenografted into mice, were studied with Doppler US. The number of intratumor vessels visualized with color Doppler US was compared with the density of microvessels and the number of vessels > 100 μm determined by histologic examination. The resistance index and the peak systolic velocities were evaluated. RESULTS. The number of intratumor vessels visualized by color Doppler US was correlated with the number of vessels >100 μm (P < 0.001) determined histologically. When vessel density was >30, intratumor vessels were always detected by color Doppler US. The resistance index and peak systolic velocities were significantly lower in intratumor than in peritumor vessels. CONCLUSIONS. Color Doppler US evaluated tumor angiogenesis accurately. Spectral analysis confirmed the low resistance of intratumor blood flow.
KW - Angiogenesis
KW - Color and pulsed Doppler ultrasonography
UR - http://www.scopus.com/inward/record.url?scp=0032973521&partnerID=8YFLogxK
U2 - 10.1097/00004424-199903000-00007
DO - 10.1097/00004424-199903000-00007
M3 - Article
C2 - 10084663
AN - SCOPUS:0032973521
SN - 0020-9996
VL - 34
SP - 194
EP - 198
JO - Investigative Radiology
JF - Investigative Radiology
IS - 3
ER -