Optimisation instrumentale de la technique de Gap-FRAP visant à discriminer différents types cellulaires selon l'activité des jonctions communicantes

J. R. Stines; M. Abbaci; W. Blondel; M. Barberi-Heyob; D. Dumas; F. Guillemin; J. Didelon

doi:10.1016/j.rbmret.2005.06.015

Optimisation instrumentale de la technique de Gap-FRAP visant à discriminer différents types cellulaires selon l'activité des jonctions communicantes

Translated title of the contribution: Instrumental optimization of Gap-FRAP technique related to gap junctions functionality for different cell types

J. R. Stines, M. Abbaci, W. Blondel, M. Barberi-Heyob, D. Dumas, F. Guillemin, J. Didelon

Research output: Contribution to journal › Article › peer-review

Abstract

Gap junctional intercellular communication has been shown to be involved in the carcinogenesis process. Gap-FRAP (Fluorescence Recovery After Photobleaching) technique could be used to estimate gap junctions functionality and their potential involvement for distinguish normal and cancer cells. We developed a sensitive microspectrofluorimetric method to characterize the kinetics of fluorescence recovery after photobleaching. We used a positive control cell line (fibroblast), a negative control (FaDu) and an intermediate (KB) head and neck cancer cell lines. Our results indicate that using our approach, the fluorescence kinetic profiles remain comparable with the method widely used by confocal microscopy.

Translated title of the contribution	Instrumental optimization of Gap-FRAP technique related to gap junctions functionality for different cell types
Original language	French
Pages (from-to)	279-281
Number of pages	3
Journal	ITBM-RBM
Volume	26
Issue number	4
DOIs	https://doi.org/10.1016/j.rbmret.2005.06.015
Publication status	Published - 1 Sept 2005
Externally published	Yes

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10.1016/j.rbmret.2005.06.015

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title = "Optimisation instrumentale de la technique de Gap-FRAP visant {\`a} discriminer diff{\'e}rents types cellulaires selon l'activit{\'e} des jonctions communicantes",

abstract = "Gap junctional intercellular communication has been shown to be involved in the carcinogenesis process. Gap-FRAP (Fluorescence Recovery After Photobleaching) technique could be used to estimate gap junctions functionality and their potential involvement for distinguish normal and cancer cells. We developed a sensitive microspectrofluorimetric method to characterize the kinetics of fluorescence recovery after photobleaching. We used a positive control cell line (fibroblast), a negative control (FaDu) and an intermediate (KB) head and neck cancer cell lines. Our results indicate that using our approach, the fluorescence kinetic profiles remain comparable with the method widely used by confocal microscopy.",

author = "Stines, {J. R.} and M. Abbaci and W. Blondel and M. Barberi-Heyob and D. Dumas and F. Guillemin and J. Didelon",

year = "2005",

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doi = "10.1016/j.rbmret.2005.06.015",

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T1 - Optimisation instrumentale de la technique de Gap-FRAP visant à discriminer différents types cellulaires selon l'activité des jonctions communicantes

AU - Stines, J. R.

AU - Abbaci, M.

AU - Blondel, W.

AU - Barberi-Heyob, M.

AU - Dumas, D.

AU - Guillemin, F.

AU - Didelon, J.

PY - 2005/9/1

Y1 - 2005/9/1

N2 - Gap junctional intercellular communication has been shown to be involved in the carcinogenesis process. Gap-FRAP (Fluorescence Recovery After Photobleaching) technique could be used to estimate gap junctions functionality and their potential involvement for distinguish normal and cancer cells. We developed a sensitive microspectrofluorimetric method to characterize the kinetics of fluorescence recovery after photobleaching. We used a positive control cell line (fibroblast), a negative control (FaDu) and an intermediate (KB) head and neck cancer cell lines. Our results indicate that using our approach, the fluorescence kinetic profiles remain comparable with the method widely used by confocal microscopy.

AB - Gap junctional intercellular communication has been shown to be involved in the carcinogenesis process. Gap-FRAP (Fluorescence Recovery After Photobleaching) technique could be used to estimate gap junctions functionality and their potential involvement for distinguish normal and cancer cells. We developed a sensitive microspectrofluorimetric method to characterize the kinetics of fluorescence recovery after photobleaching. We used a positive control cell line (fibroblast), a negative control (FaDu) and an intermediate (KB) head and neck cancer cell lines. Our results indicate that using our approach, the fluorescence kinetic profiles remain comparable with the method widely used by confocal microscopy.

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DO - 10.1016/j.rbmret.2005.06.015

M3 - Article

AN - SCOPUS:24144472321

SN - 1297-9562

VL - 26

SP - 279

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JO - ITBM-RBM

JF - ITBM-RBM

IS - 4

ER -

Optimisation instrumentale de la technique de Gap-FRAP visant à discriminer différents types cellulaires selon l'activité des jonctions communicantes

Abstract

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