TY - JOUR
T1 - p-ERK1/2 is a predictive factor of response to erythropoiesis-stimulating agents in low/int-1 myelodysplastic syndromes
AU - Frisan, Emilie
AU - Pawlikowska, Patrycja
AU - Pierre-Eugène, Cecile
AU - Viallon, Vivian
AU - Gibault, Laure
AU - Park, Sophie
AU - Mayeux, Patrick
AU - Dreyfus, François
AU - Porteu, Françoise
AU - Fontenay, Michaëla
PY - 2010/11/1
Y1 - 2010/11/1
N2 - Serum erythropoietin level less than 100U/L and a transfusion requirement of less than 2 units per month are the best predictive factors for response to treatment by erythropoiesis-stimulating agents in low/int-1 myelodysplastic syndromes. To investigate the factors influencing the response to erythropoiesis-stimulating agents, we enrolled 127 low/int-1 myelodysplastic syndrome patients at diagnosis in a biological study of erythropoiesis. The 54 nonresponders had a significantly lower number of burst-forming unit-erythroid and colony-forming unit-erythroid than responders. Erythropoietin-dependent proliferation and survival, and phospho (p)-ERK1/2 expression in steady state and after erythropoietin stimulation were defective in cultured erythroblasts. By flow cytometry, p-ERK1/2 was significantly lower in bone marrow CD45-/CD71+/GPA- cells from non-responders compared to responders or controls. Receiver Operator Characteristic curve analysis showed that this flow cytometry test was a sensitive biomarker for predicting the response to erythropoiesis-stimulating agents.
AB - Serum erythropoietin level less than 100U/L and a transfusion requirement of less than 2 units per month are the best predictive factors for response to treatment by erythropoiesis-stimulating agents in low/int-1 myelodysplastic syndromes. To investigate the factors influencing the response to erythropoiesis-stimulating agents, we enrolled 127 low/int-1 myelodysplastic syndrome patients at diagnosis in a biological study of erythropoiesis. The 54 nonresponders had a significantly lower number of burst-forming unit-erythroid and colony-forming unit-erythroid than responders. Erythropoietin-dependent proliferation and survival, and phospho (p)-ERK1/2 expression in steady state and after erythropoietin stimulation were defective in cultured erythroblasts. By flow cytometry, p-ERK1/2 was significantly lower in bone marrow CD45-/CD71+/GPA- cells from non-responders compared to responders or controls. Receiver Operator Characteristic curve analysis showed that this flow cytometry test was a sensitive biomarker for predicting the response to erythropoiesis-stimulating agents.
KW - Erythropoiesis-stimulating agents
KW - MAPkinase
KW - Myelodysplastic syndromes
KW - Red cells
UR - http://www.scopus.com/inward/record.url?scp=78149250055&partnerID=8YFLogxK
U2 - 10.3324/haematol.2010.024349
DO - 10.3324/haematol.2010.024349
M3 - Article
C2 - 20823131
AN - SCOPUS:78149250055
SN - 0390-6078
VL - 95
SP - 1964
EP - 1968
JO - Haematologica
JF - Haematologica
IS - 11
ER -