p27(KiP1) overexpression inhibits the growth and doxorubicin sensitivity of HT29 human colon cancer cells in vivo

Marie thérèse Dimanche-Boitrel; Olivier Micheau; Damien France; Arlette Hammann; Olivier Duchamp; Philippe Genne; Eric Solary

p27(KiP1) overexpression inhibits the growth and doxorubicin sensitivity of HT29 human colon cancer cells in vivo

Marie thérèse Dimanche-Boitrel, Olivier Micheau, Damien France, Arlette Hammann, Olivier Duchamp, Philippe Genne, Eric Solary

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

We have previously shown that p27(KiP1) plays a role in the tumor cell resistance of HT29 confluent monolayers to cytotoxic drugs in vitro. To determine whether p27(KiP1) was a resistance factor to cytotoxic drugs in vivo we tested the effect of doxorubicin on p27(KiP1)-overexpressing HT29 tumors in nude mice. In this study we show that ectopic overexpression of p27(KiP1) in HT29 human colon cancer cells decreases their tumorigenicity in vivo in nude mice. This decreased tumor growth was associated with increased p27(KiP1) protein expression studied by Western blotting in tumor extracts. Interestingly, the overexpressing-p27(KiP1) tumors were significantly more resistant to intraveneous doxorubicin treatment than the control tumors. These results indicate that p27(KiP1), which delays tumor growth could also increase tumor resistance to cytotoxic drugs in vivo.

Original language	English
Pages (from-to)	849-852
Number of pages	4
Journal	Anticancer Research
Volume	20
Issue number	2 A
Publication status	Published - 1 Jan 2000

Keywords

Chemoresistance
Colon cancer
Doxorubicin
Nude mice
p27(KiP1)

Cite this

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title = "p27(KiP1) overexpression inhibits the growth and doxorubicin sensitivity of HT29 human colon cancer cells in vivo",

abstract = "We have previously shown that p27(KiP1) plays a role in the tumor cell resistance of HT29 confluent monolayers to cytotoxic drugs in vitro. To determine whether p27(KiP1) was a resistance factor to cytotoxic drugs in vivo we tested the effect of doxorubicin on p27(KiP1)-overexpressing HT29 tumors in nude mice. In this study we show that ectopic overexpression of p27(KiP1) in HT29 human colon cancer cells decreases their tumorigenicity in vivo in nude mice. This decreased tumor growth was associated with increased p27(KiP1) protein expression studied by Western blotting in tumor extracts. Interestingly, the overexpressing-p27(KiP1) tumors were significantly more resistant to intraveneous doxorubicin treatment than the control tumors. These results indicate that p27(KiP1), which delays tumor growth could also increase tumor resistance to cytotoxic drugs in vivo.",

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T1 - p27(KiP1) overexpression inhibits the growth and doxorubicin sensitivity of HT29 human colon cancer cells in vivo

AU - Dimanche-Boitrel, Marie thérèse

AU - Micheau, Olivier

AU - France, Damien

AU - Hammann, Arlette

AU - Duchamp, Olivier

AU - Genne, Philippe

AU - Solary, Eric

PY - 2000/1/1

Y1 - 2000/1/1

N2 - We have previously shown that p27(KiP1) plays a role in the tumor cell resistance of HT29 confluent monolayers to cytotoxic drugs in vitro. To determine whether p27(KiP1) was a resistance factor to cytotoxic drugs in vivo we tested the effect of doxorubicin on p27(KiP1)-overexpressing HT29 tumors in nude mice. In this study we show that ectopic overexpression of p27(KiP1) in HT29 human colon cancer cells decreases their tumorigenicity in vivo in nude mice. This decreased tumor growth was associated with increased p27(KiP1) protein expression studied by Western blotting in tumor extracts. Interestingly, the overexpressing-p27(KiP1) tumors were significantly more resistant to intraveneous doxorubicin treatment than the control tumors. These results indicate that p27(KiP1), which delays tumor growth could also increase tumor resistance to cytotoxic drugs in vivo.

AB - We have previously shown that p27(KiP1) plays a role in the tumor cell resistance of HT29 confluent monolayers to cytotoxic drugs in vitro. To determine whether p27(KiP1) was a resistance factor to cytotoxic drugs in vivo we tested the effect of doxorubicin on p27(KiP1)-overexpressing HT29 tumors in nude mice. In this study we show that ectopic overexpression of p27(KiP1) in HT29 human colon cancer cells decreases their tumorigenicity in vivo in nude mice. This decreased tumor growth was associated with increased p27(KiP1) protein expression studied by Western blotting in tumor extracts. Interestingly, the overexpressing-p27(KiP1) tumors were significantly more resistant to intraveneous doxorubicin treatment than the control tumors. These results indicate that p27(KiP1), which delays tumor growth could also increase tumor resistance to cytotoxic drugs in vivo.

KW - Chemoresistance

KW - Colon cancer

KW - Doxorubicin

KW - Nude mice

KW - p27(KiP1)

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M3 - Article

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AN - SCOPUS:0033625689

SN - 0250-7005

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JO - Anticancer Research

JF - Anticancer Research

IS - 2 A

ER -

p27(KiP1) overexpression inhibits the growth and doxorubicin sensitivity of HT29 human colon cancer cells in vivo

Abstract

Keywords

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