Plasmacytoid dendritic cells develop from Ly6D+ lymphoid progenitors distinct from the myeloid lineage

Regine J. Dress; Charles Antoine Dutertre; Amir Giladi; Andreas Schlitzer; Ivy Low; Nurhidaya Binte Shadan; Alicia Tay; Josephine Lum; Muhammad Faris Bin Mohd Kairi; You Yi Hwang; Etienne Becht; Yang Cheng; Marion Chevrier; Anis Larbi; Evan W. Newell; Ido Amit; Jinmiao Chen; Florent Ginhoux

doi:10.1038/s41590-019-0420-3

Plasmacytoid dendritic cells develop from Ly6D⁺ lymphoid progenitors distinct from the myeloid lineage

Regine J. Dress, Charles Antoine Dutertre, Amir Giladi, Andreas Schlitzer, Ivy Low, Nurhidaya Binte Shadan, Alicia Tay, Josephine Lum, Muhammad Faris Bin Mohd Kairi, You Yi Hwang, Etienne Becht, Yang Cheng, Marion Chevrier, Anis Larbi, Evan W. Newell, Ido Amit, Jinmiao Chen, Florent Ginhoux

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154 Citations (Scopus)

Abstract

Dendritic cells (DC) are currently classified as conventional DCs (cDCs) and plasmacytoid DCs (pDCs). Through a combination of single-cell transcriptomic analysis, mass cytometry, in vivo fate mapping and in vitro clonal assays, here we show that, at the single-cell level, the priming of mouse hematopoietic progenitor cells toward the pDC lineage occurs at the common lymphoid progenitor stage, indicative of early divergence of the pDC and cDC lineages. We found the transcriptional signature of a pDC precursor stage, defined here, in the IL-7Rα⁺ common lymphoid progenitor population and identified Ly6D, IL-7Rα, CD81 and CD2 as key markers of pDC differentiation, which distinguish pDC precursors from cDC precursors. In conclusion, pDCs developed in the bone marrow from a Ly6D^hiCD2^hi lymphoid progenitor cell and differentiated independently of the myeloid cDC lineage.

Original language	English
Pages (from-to)	852-864
Number of pages	13
Journal	Nature Immunology
Volume	20
Issue number	7
DOIs	https://doi.org/10.1038/s41590-019-0420-3
Publication status	Published - 1 Jul 2019
Externally published	Yes

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Dress, R. J., Dutertre, C. A., Giladi, A., Schlitzer, A., Low, I., Shadan, N. B., Tay, A., Lum, J., Kairi, M. F. B. M., Hwang, Y. Y., Becht, E., Cheng, Y., Chevrier, M., Larbi, A., Newell, E. W., Amit, I., Chen, J., & Ginhoux, F. (2019). Plasmacytoid dendritic cells develop from Ly6D⁺ lymphoid progenitors distinct from the myeloid lineage. Nature Immunology, 20(7), 852-864. https://doi.org/10.1038/s41590-019-0420-3

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title = "Plasmacytoid dendritic cells develop from Ly6D+ lymphoid progenitors distinct from the myeloid lineage",

abstract = "Dendritic cells (DC) are currently classified as conventional DCs (cDCs) and plasmacytoid DCs (pDCs). Through a combination of single-cell transcriptomic analysis, mass cytometry, in vivo fate mapping and in vitro clonal assays, here we show that, at the single-cell level, the priming of mouse hematopoietic progenitor cells toward the pDC lineage occurs at the common lymphoid progenitor stage, indicative of early divergence of the pDC and cDC lineages. We found the transcriptional signature of a pDC precursor stage, defined here, in the IL-7Rα+ common lymphoid progenitor population and identified Ly6D, IL-7Rα, CD81 and CD2 as key markers of pDC differentiation, which distinguish pDC precursors from cDC precursors. In conclusion, pDCs developed in the bone marrow from a Ly6DhiCD2hi lymphoid progenitor cell and differentiated independently of the myeloid cDC lineage.",

author = "Dress, {Regine J.} and Dutertre, {Charles Antoine} and Amir Giladi and Andreas Schlitzer and Ivy Low and Shadan, {Nurhidaya Binte} and Alicia Tay and Josephine Lum and Kairi, {Muhammad Faris Bin Mohd} and Hwang, {You Yi} and Etienne Becht and Yang Cheng and Marion Chevrier and Anis Larbi and Newell, {Evan W.} and Ido Amit and Jinmiao Chen and Florent Ginhoux",

note = "Publisher Copyright: {\textcopyright} 2019, The Author(s), under exclusive licence to Springer Nature America, Inc.",

year = "2019",

month = jul,

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doi = "10.1038/s41590-019-0420-3",

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Dress, RJ, Dutertre, CA, Giladi, A, Schlitzer, A, Low, I, Shadan, NB, Tay, A, Lum, J, Kairi, MFBM, Hwang, YY, Becht, E, Cheng, Y, Chevrier, M, Larbi, A, Newell, EW, Amit, I, Chen, J & Ginhoux, F 2019, 'Plasmacytoid dendritic cells develop from Ly6D⁺ lymphoid progenitors distinct from the myeloid lineage', Nature Immunology, vol. 20, no. 7, pp. 852-864. https://doi.org/10.1038/s41590-019-0420-3

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T1 - Plasmacytoid dendritic cells develop from Ly6D+ lymphoid progenitors distinct from the myeloid lineage

AU - Dress, Regine J.

AU - Dutertre, Charles Antoine

AU - Giladi, Amir

AU - Schlitzer, Andreas

AU - Low, Ivy

AU - Shadan, Nurhidaya Binte

AU - Tay, Alicia

AU - Lum, Josephine

AU - Kairi, Muhammad Faris Bin Mohd

AU - Hwang, You Yi

AU - Becht, Etienne

AU - Cheng, Yang

AU - Chevrier, Marion

AU - Larbi, Anis

AU - Newell, Evan W.

AU - Amit, Ido

AU - Chen, Jinmiao

AU - Ginhoux, Florent

PY - 2019/7/1

Y1 - 2019/7/1

N2 - Dendritic cells (DC) are currently classified as conventional DCs (cDCs) and plasmacytoid DCs (pDCs). Through a combination of single-cell transcriptomic analysis, mass cytometry, in vivo fate mapping and in vitro clonal assays, here we show that, at the single-cell level, the priming of mouse hematopoietic progenitor cells toward the pDC lineage occurs at the common lymphoid progenitor stage, indicative of early divergence of the pDC and cDC lineages. We found the transcriptional signature of a pDC precursor stage, defined here, in the IL-7Rα+ common lymphoid progenitor population and identified Ly6D, IL-7Rα, CD81 and CD2 as key markers of pDC differentiation, which distinguish pDC precursors from cDC precursors. In conclusion, pDCs developed in the bone marrow from a Ly6DhiCD2hi lymphoid progenitor cell and differentiated independently of the myeloid cDC lineage.

AB - Dendritic cells (DC) are currently classified as conventional DCs (cDCs) and plasmacytoid DCs (pDCs). Through a combination of single-cell transcriptomic analysis, mass cytometry, in vivo fate mapping and in vitro clonal assays, here we show that, at the single-cell level, the priming of mouse hematopoietic progenitor cells toward the pDC lineage occurs at the common lymphoid progenitor stage, indicative of early divergence of the pDC and cDC lineages. We found the transcriptional signature of a pDC precursor stage, defined here, in the IL-7Rα+ common lymphoid progenitor population and identified Ly6D, IL-7Rα, CD81 and CD2 as key markers of pDC differentiation, which distinguish pDC precursors from cDC precursors. In conclusion, pDCs developed in the bone marrow from a Ly6DhiCD2hi lymphoid progenitor cell and differentiated independently of the myeloid cDC lineage.

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Plasmacytoid dendritic cells develop from Ly6D+ lymphoid progenitors distinct from the myeloid lineage

Abstract

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Plasmacytoid dendritic cells develop from Ly6D⁺ lymphoid progenitors distinct from the myeloid lineage