Quantitative high-performance liquid chromatographic determination of acrolein in plasma after derivatization with Luminarin(®) 3

A. Paci; A. Rieutord; D. Guillaume; F. Traoré; J. Ropenga; H. P. Husson; F. Brion

doi:10.1016/S0378-4347(99)00485-5

Quantitative high-performance liquid chromatographic determination of acrolein in plasma after derivatization with Luminarin(®) 3

A. Paci, A. Rieutord, D. Guillaume, F. Traoré, J. Ropenga, H. P. Husson, F. Brion

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

A rapid, sensitive and specific high-performance liquid chromatographic method for the quantification of acrolein (1), one of the toxic metabolites of oxazaphosphorine alkylating agents (cyclophosphamide and ifosfamide) was developed. Condensation of acrolein with Luminarin(®) 3 afforded a fluorescent derivative that could be specifically detected and quantified. Chromatographic conditions involved a C₁₈ RP column Uptisphere and a gradient elution system to optimize resolution and time analysis. The method showed high sensitivity with a limit of detection of 100 pmol/ml and a limit of quantification of 300 pmol/ml. This technique is particularly suitable for pharmacokinetic studies on plasma of oxazaphosphorine-receiving patients. Copyright (C) 2000 Elsevier Science B.V.

Original language	English
Pages (from-to)	239-246
Number of pages	8
Journal	Journal of Chromatography B: Biomedical Sciences and Applications
Volume	739
Issue number	2
DOIs	https://doi.org/10.1016/S0378-4347(99)00485-5
Publication status	Published - 10 Mar 2000
Externally published	Yes

Keywords

Acrolein
Cyclophosphamide
Ifosfamide
Luminarin

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10.1016/S0378-4347(99)00485-5

Cite this

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title = "Quantitative high-performance liquid chromatographic determination of acrolein in plasma after derivatization with Luminarin({\textregistered}) 3",

abstract = "A rapid, sensitive and specific high-performance liquid chromatographic method for the quantification of acrolein (1), one of the toxic metabolites of oxazaphosphorine alkylating agents (cyclophosphamide and ifosfamide) was developed. Condensation of acrolein with Luminarin({\textregistered}) 3 afforded a fluorescent derivative that could be specifically detected and quantified. Chromatographic conditions involved a C18 RP column Uptisphere and a gradient elution system to optimize resolution and time analysis. The method showed high sensitivity with a limit of detection of 100 pmol/ml and a limit of quantification of 300 pmol/ml. This technique is particularly suitable for pharmacokinetic studies on plasma of oxazaphosphorine-receiving patients. Copyright (C) 2000 Elsevier Science B.V.",

keywords = "Acrolein, Cyclophosphamide, Ifosfamide, Luminarin",

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T1 - Quantitative high-performance liquid chromatographic determination of acrolein in plasma after derivatization with Luminarin(®) 3

AU - Paci, A.

AU - Rieutord, A.

AU - Guillaume, D.

AU - Traoré, F.

AU - Ropenga, J.

AU - Husson, H. P.

AU - Brion, F.

PY - 2000/3/10

Y1 - 2000/3/10

N2 - A rapid, sensitive and specific high-performance liquid chromatographic method for the quantification of acrolein (1), one of the toxic metabolites of oxazaphosphorine alkylating agents (cyclophosphamide and ifosfamide) was developed. Condensation of acrolein with Luminarin(®) 3 afforded a fluorescent derivative that could be specifically detected and quantified. Chromatographic conditions involved a C18 RP column Uptisphere and a gradient elution system to optimize resolution and time analysis. The method showed high sensitivity with a limit of detection of 100 pmol/ml and a limit of quantification of 300 pmol/ml. This technique is particularly suitable for pharmacokinetic studies on plasma of oxazaphosphorine-receiving patients. Copyright (C) 2000 Elsevier Science B.V.

AB - A rapid, sensitive and specific high-performance liquid chromatographic method for the quantification of acrolein (1), one of the toxic metabolites of oxazaphosphorine alkylating agents (cyclophosphamide and ifosfamide) was developed. Condensation of acrolein with Luminarin(®) 3 afforded a fluorescent derivative that could be specifically detected and quantified. Chromatographic conditions involved a C18 RP column Uptisphere and a gradient elution system to optimize resolution and time analysis. The method showed high sensitivity with a limit of detection of 100 pmol/ml and a limit of quantification of 300 pmol/ml. This technique is particularly suitable for pharmacokinetic studies on plasma of oxazaphosphorine-receiving patients. Copyright (C) 2000 Elsevier Science B.V.

KW - Acrolein

KW - Cyclophosphamide

KW - Ifosfamide

KW - Luminarin

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U2 - 10.1016/S0378-4347(99)00485-5

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JO - Journal of Chromatography B: Biomedical Sciences and Applications

JF - Journal of Chromatography B: Biomedical Sciences and Applications

IS - 2

ER -

Quantitative high-performance liquid chromatographic determination of acrolein in plasma after derivatization with Luminarin(®) 3

Abstract

Keywords

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