TY - JOUR
T1 - Rab27a and Rab27b control different steps of the exosome secretion pathway
AU - Ostrowski, Matias
AU - Carmo, Nuno B.
AU - Krumeich, Sophie
AU - Fanget, Isabelle
AU - Raposo, Graça
AU - Savina, Ariel
AU - Moita, Catarina F.
AU - Schauer, Kristine
AU - Hume, Alistair N.
AU - Freitas, Rui P.
AU - Goud, Bruno
AU - Benaroch, Philippe
AU - Hacohen, Nir
AU - Fukuda, Mitsunori
AU - Desnos, Claire
AU - Seabra, Miguel C.
AU - Darchen, François
AU - Amigorena, Sebastian
AU - Moita, Luis F.
AU - Thery, Clotilde
N1 - Funding Information:
This work was supported by post-doctoral salaries from Institut National du Cancer and Institut Curie to M.O., grants from Association pour la Recherche sur le Cancer and Fondation de France to C.T., and from the Agence Nationale de la Recherche to F.D. (ANR-06-BLAN-0211-02). L.F.M. is a Young Investigator from the Human Frontier Science Program and receives support from Fundação Luso-Americana para o Desenvolvimento and Fundação para a Ciência e a Tecnologia (PTDC/SAU-MII/69280/2006 and PTDC/SAU-MII/78333/2006). We thank PICT IbiSA Imaging Facility at Curie Institute, V. Racine and J.-B. Sibarita for providing a copy of their multidimensional image analysis program, I. Hurbain for help in electron microscopy quantification, P. Simões and M. H. Raquel for some of the lentiviral preparations, T. Tolmachova for bones of Rab27-knockout mice, J. Mordoh for providing an anti-CD63 FC-5.01 antibody and R. Allan for critical reading of the manuscript.
PY - 2010/1/1
Y1 - 2010/1/1
N2 - Exosomes are secreted membrane vesicles that share structural and biochemical characteristics with intraluminal vesicles of multivesicular endosomes (MVEs). Exosomes could be involved in intercellular communication and in the pathogenesis of infectious and degenerative diseases. The molecular mechanisms of exosome biogenesis and secretion are, however, poorly understood. Using an RNA interference (RNAi) screen, we identified five Rab GTPases that promote exosome secretion in HeLa cells. Among these, Rab27a and Rab27b were found to function in MVE docking at the plasma membrane. The size of MVEs was strongly increased by Rab27a silencing, whereas MVEs were redistributed towards the perinuclear region upon Rab27b silencing. Thus, the two Rab27 isoforms have different roles in the exosomal pathway. In addition, silencing two known Rab27 effectors, Slp4 (also known as SYTL4, synaptotagmin-like 4) and Slac2b (also known as EXPH5, exophilin 5), inhibited exosome secretion and phenocopied silencing of Rab27a and Rab27b, respectively. Our results therefore strengthen the link between MVEs and exosomes, and introduce ways of manipulating exosome secretion in vivo.
AB - Exosomes are secreted membrane vesicles that share structural and biochemical characteristics with intraluminal vesicles of multivesicular endosomes (MVEs). Exosomes could be involved in intercellular communication and in the pathogenesis of infectious and degenerative diseases. The molecular mechanisms of exosome biogenesis and secretion are, however, poorly understood. Using an RNA interference (RNAi) screen, we identified five Rab GTPases that promote exosome secretion in HeLa cells. Among these, Rab27a and Rab27b were found to function in MVE docking at the plasma membrane. The size of MVEs was strongly increased by Rab27a silencing, whereas MVEs were redistributed towards the perinuclear region upon Rab27b silencing. Thus, the two Rab27 isoforms have different roles in the exosomal pathway. In addition, silencing two known Rab27 effectors, Slp4 (also known as SYTL4, synaptotagmin-like 4) and Slac2b (also known as EXPH5, exophilin 5), inhibited exosome secretion and phenocopied silencing of Rab27a and Rab27b, respectively. Our results therefore strengthen the link between MVEs and exosomes, and introduce ways of manipulating exosome secretion in vivo.
UR - http://www.scopus.com/inward/record.url?scp=79953136097&partnerID=8YFLogxK
U2 - 10.1038/ncb2000
DO - 10.1038/ncb2000
M3 - Article
C2 - 19966785
AN - SCOPUS:79953136097
SN - 1465-7392
VL - 12
SP - 19
EP - 30
JO - Nature Cell Biology
JF - Nature Cell Biology
IS - 1
ER -