TY - JOUR
T1 - Serum metabolomic adaptations following a 12-week high-intensity interval training combined to citrulline supplementation in obese older adults
AU - Youssef, Layale
AU - Durand, Sylvère
AU - Aprahamian, Fanny
AU - Lefevre, Deborah
AU - Bourgin, Mélanie
AU - Maiuri, Maria Chiara
AU - Dulac, Maude
AU - Hajj-Boutros, Guy
AU - Marcangeli, Vincent
AU - Buckinx, Fanny
AU - Peyrusqué, Eva
AU - Morais, José A.
AU - Gaudreau, Pierrette
AU - Gouspillou, Gilles
AU - Kroemer, Guido
AU - Aubertin-Leheudre, Mylène
AU - Noirez, Philippe
N1 - Publisher Copyright:
© 2023 European College of Sport Science.
PY - 2023/1/1
Y1 - 2023/1/1
N2 - Physical activity and nutrition play important roles in preventing adverse health outcomes that accompany aging. It has been shown that high-intensity interval training (HIIT) combined with citrulline (CIT) supplementation can improve physical and functional capacities. The aim of this study was to evaluate serum metabolites following a 12-week HIIT combined or not with CIT in obese older adults, and to correlate the metabolic changes with clinico-biological parameters changes. Eighty-six obese older adults completed a 12-week HIIT program combined with a 10 g daily supplementation of either CIT or placebo (PLA) during a double-blinded randomized interventional trial. Only participants with blood samples at T0 (before the intervention) and/or T12 (after the intervention) were included in our sub-analysis (HIIT-PLA-T0: n = 44 and HIIT-PLA-T12: n = 28; HIIT-CIT-T0: n = 39 and HIIT-CIT-T12: n = 42). Serum samples were analyzed by different liquid or gas phase chromatography methods coupled to mass spectrometry. Among the identified metabolites, 44 changed significantly following the 12-week intervention (Time effect), and 10 of them were more affected when HIIT was combined with CIT (Time × Supp effect). Arginine increased significantly due to the 12-week intervention. Correlation analyses demonstrated that decreased triglyceride (TG) (16:1/18:1/16:0) and aspartic acid significantly correlated with a reduction of adiposity-related parameters (fat mass, leg lean mass, leptin, total triglycerides and low-density lipoprotein). Arginine, TG (16:1/18:1/16:0) and aspartic acid might constitute biomarkers of cardiometabolic health and adiposity. Further studies are needed to confirm these associations and understand the underlying mechanisms. Highlights A 12-week intervention involving high-intensity interval training (HIIT) with or without citrulline (CIT) supplementation induced adaptations in the serum metabolome of obese older adults through significant changes in 44 metabolites. Changes in 23 metabolites were observed when a CIT supplementation was administered along with a 12-week HIIT intervention. TG (16:1/18:1/16:0) correlated with several adiposity parameters including leptin, triglycerides, legs lean mass. Aspartic acid correlated with several adiposity parameters including leptin, LDL cholesterol as well as android, arms and trunk fat mass.
AB - Physical activity and nutrition play important roles in preventing adverse health outcomes that accompany aging. It has been shown that high-intensity interval training (HIIT) combined with citrulline (CIT) supplementation can improve physical and functional capacities. The aim of this study was to evaluate serum metabolites following a 12-week HIIT combined or not with CIT in obese older adults, and to correlate the metabolic changes with clinico-biological parameters changes. Eighty-six obese older adults completed a 12-week HIIT program combined with a 10 g daily supplementation of either CIT or placebo (PLA) during a double-blinded randomized interventional trial. Only participants with blood samples at T0 (before the intervention) and/or T12 (after the intervention) were included in our sub-analysis (HIIT-PLA-T0: n = 44 and HIIT-PLA-T12: n = 28; HIIT-CIT-T0: n = 39 and HIIT-CIT-T12: n = 42). Serum samples were analyzed by different liquid or gas phase chromatography methods coupled to mass spectrometry. Among the identified metabolites, 44 changed significantly following the 12-week intervention (Time effect), and 10 of them were more affected when HIIT was combined with CIT (Time × Supp effect). Arginine increased significantly due to the 12-week intervention. Correlation analyses demonstrated that decreased triglyceride (TG) (16:1/18:1/16:0) and aspartic acid significantly correlated with a reduction of adiposity-related parameters (fat mass, leg lean mass, leptin, total triglycerides and low-density lipoprotein). Arginine, TG (16:1/18:1/16:0) and aspartic acid might constitute biomarkers of cardiometabolic health and adiposity. Further studies are needed to confirm these associations and understand the underlying mechanisms. Highlights A 12-week intervention involving high-intensity interval training (HIIT) with or without citrulline (CIT) supplementation induced adaptations in the serum metabolome of obese older adults through significant changes in 44 metabolites. Changes in 23 metabolites were observed when a CIT supplementation was administered along with a 12-week HIIT intervention. TG (16:1/18:1/16:0) correlated with several adiposity parameters including leptin, triglycerides, legs lean mass. Aspartic acid correlated with several adiposity parameters including leptin, LDL cholesterol as well as android, arms and trunk fat mass.
KW - HIIT
KW - aging
KW - exercise
KW - nutrition
KW - obesity
UR - http://www.scopus.com/inward/record.url?scp=85159937557&partnerID=8YFLogxK
U2 - 10.1080/17461391.2023.2213185
DO - 10.1080/17461391.2023.2213185
M3 - Article
C2 - 37161876
AN - SCOPUS:85159937557
SN - 1746-1391
VL - 23
SP - 2157
EP - 2169
JO - European Journal of Sport Science
JF - European Journal of Sport Science
IS - 11
ER -