Side-by-side comparison of flow cytometry and immunohistochemistry for detection of calreticulin exposure in the course of immunogenic cell death

Lenka Kasikova, Iva Truxova, Isabelle Cremer, Catherine Sautes-Fridman, Oliver Kepp, Guido Kroemer, Radek Spisek, Jitka Fucikova

    Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

    3 Citations (Scopus)

    Abstract

    Immunogenic cell death (ICD), a functionally peculiar type of apoptosis, represents a unique way to deliver danger-associated molecular patterns (DAMPs) to the tumor microenvironment. Once emitted by dying cancer cells, DAMPs orchestrate antigen-specific immune responses by acting on both innate and adaptive components of the immune system. Accumulating preclinical and clinical evidence indicates that one of these DAMPs, calreticulin (CALR) represents a novel powerful prognostic biomarker, reflecting the activation of a clinically relevant anticancer immune response in different cancer malignancies. Therefore, the assessment of CALR emission can provide a therapeutic tool for the stratification of cancer patients and the identification of individuals that are intrinsically capable to respond to a particular treatment. Here we describe methods for the quantification of CALR exposure in the tumor microenvironment of cancer patients by flow cytometry and immunohistochemistry.

    Original languageEnglish
    Title of host publicationTumor Immunology and Immunotherapy - Cellular Methods Part B
    EditorsLorenzo Galluzzi, Nils-Petter Rudqvist
    PublisherAcademic Press Inc.
    Pages15-25
    Number of pages11
    ISBN (Print)9780128186756
    DOIs
    Publication statusPublished - 1 Jan 2020

    Publication series

    NameMethods in Enzymology
    Volume632
    ISSN (Print)0076-6879
    ISSN (Electronic)1557-7988

    Keywords

    • Calreticulin
    • Cancer prognosis
    • Flow cytometry
    • Immunogenic cell death
    • Immunohistochemistry

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