Striatal PSA-NCAM+ precursor cells from the newborn rat express functional glycine receptors

Laurent Nguyen, Brigitte Malgrange, Ingrid Breuskin, François Lallemend, Grégory Hans, Gustave Moonen, Shibeshih Belachew, Jean Michel Rigo

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4 Citations (Scopus)

Abstract

Immunocytochemical analysis showed that ionotropic glycine receptors are expressed in neurogenic progenitors purified from the newborn rat striatum and expressing the polysialylated form of the neural cell adhesion molecule, both in vitro and in situ. To ascertain whether glycine receptors were functional in vitro, whole-cell patch-clamp recordings demonstrated that glycine triggers inward strychnine-sensitive currents in the majority of these cells. Moreover, we found that glycine receptors expressed by these neurogenic progenitors display intermediate electrophysiological characteristics between those of glycine receptors expressed by neural stem cells and by mature interneurons from the rat striatum. Altogether, the present data show that functional strychnine-sensitive glycine receptors are expressed in neurogenic progenitors purified from the newborn rat striatum.

Original languageEnglish
Pages (from-to)583-587
Number of pages5
JournalNeuroReport
Volume15
Issue number4
DOIs
Publication statusPublished - 22 Mar 2004
Externally publishedYes

Keywords

  • Glycine receptors
  • Immunocytochemistry
  • Neuroblast
  • Newborn rat
  • PSA-NCAM
  • Strychnine
  • Whole-cell patch-clamp

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