Transitional premonocytes emerge in the periphery for host defense against bacterial infections

Ye Chean Teh; Ming Yao Chooi; Dehua Liu; Immanuel Kwok; Ghee Chuan Lai; Liyana Ayub Ow Yong; Melissa Ng; Jackson L.Y. Li; Yingrou Tan; Maximilien Evrard; Leonard Tan; Ka Hang Liong; Keith Leong; Chi Ching Goh; Andrew Y.J. Chan; Nurhidaya Binte Shadan; Chinmay Kumar Mantri; You Yi Hwang; Hui Cheng; Tao Cheng; Weimiao Yu; Hong Liang Tey; Anis Larbi; Ashley St John; Veronique Angeli; Christiane Ruedl; Bernett Lee; Florent Ginhoux; Swaine L. Chen; Lai Guan Ng; Jeak Ling Ding; Shu Zhen Chong

doi:10.1126/sciadv.abj4641

Transitional premonocytes emerge in the periphery for host defense against bacterial infections

Ye Chean Teh, Ming Yao Chooi, Dehua Liu, Immanuel Kwok, Ghee Chuan Lai, Liyana Ayub Ow Yong, Melissa Ng, Jackson L.Y. Li, Yingrou Tan, Maximilien Evrard, Leonard Tan, Ka Hang Liong, Keith Leong, Chi Ching Goh, Andrew Y.J. Chan, Nurhidaya Binte Shadan, Chinmay Kumar Mantri, You Yi Hwang, Hui Cheng, Tao ChengWeimiao Yu, Hong Liang Tey, Anis Larbi, Ashley St John, Veronique Angeli, Christiane Ruedl, Bernett Lee, Florent Ginhoux, Swaine L. Chen, Lai Guan Ng, Jeak Ling Ding, Shu Zhen Chong

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Circulating Ly6Chi monocytes often undergo cellular death upon exhaustion of their antibacterial effector functions, which limits their capacity for subsequent macrophage differentiation. This shrouds the understanding on how the host replaces the tissue-resident macrophage niche effectively during bacterial invasion to avert infection morbidity. Here, we show that proliferating transitional premonocytes (TpMos), an immediate precursor of mature Ly6Chi monocytes (MatMos), were mobilized into the periphery in response to acute bacterial infection and sepsis. TpMos were less susceptible to apoptosis and served as the main source of macrophage replenishment when MatMos were vulnerable toward bacteria-induced cellular death. Furthermore, TpMo and its derived macrophages contributed to host defense by balancing the proinflammatory cytokine response of MatMos. Consequently, adoptive transfer of TpMos improved the survival outcome of lethal sepsis. Our findings hence highlight a protective role for TpMos during bacterial infections and their contribution toward monocyte-derived macrophage heterogeneity in distinct disease outcomes.

Original language	English
Article number	eabj4641
Journal	Science Advances
Volume	8
Issue number	9
DOIs	https://doi.org/10.1126/sciadv.abj4641
Publication status	Published - 1 Mar 2022
Externally published	Yes

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Teh, Y. C., Chooi, M. Y., Liu, D., Kwok, I., Lai, G. C., Yong, L. A. O., Ng, M., Li, J. L. Y., Tan, Y., Evrard, M., Tan, L., Liong, K. H., Leong, K., Goh, C. C., Chan, A. Y. J., Shadan, N. B., Mantri, C. K., Hwang, Y. Y., Cheng, H., ... Chong, S. Z. (2022). Transitional premonocytes emerge in the periphery for host defense against bacterial infections. Science Advances, 8(9), Article eabj4641. https://doi.org/10.1126/sciadv.abj4641

@article{c3cd0fa6983b4cc7be5080e62edaa12b,

title = "Transitional premonocytes emerge in the periphery for host defense against bacterial infections",

abstract = "Circulating Ly6Chi monocytes often undergo cellular death upon exhaustion of their antibacterial effector functions, which limits their capacity for subsequent macrophage differentiation. This shrouds the understanding on how the host replaces the tissue-resident macrophage niche effectively during bacterial invasion to avert infection morbidity. Here, we show that proliferating transitional premonocytes (TpMos), an immediate precursor of mature Ly6Chi monocytes (MatMos), were mobilized into the periphery in response to acute bacterial infection and sepsis. TpMos were less susceptible to apoptosis and served as the main source of macrophage replenishment when MatMos were vulnerable toward bacteria-induced cellular death. Furthermore, TpMo and its derived macrophages contributed to host defense by balancing the proinflammatory cytokine response of MatMos. Consequently, adoptive transfer of TpMos improved the survival outcome of lethal sepsis. Our findings hence highlight a protective role for TpMos during bacterial infections and their contribution toward monocyte-derived macrophage heterogeneity in distinct disease outcomes.",

author = "Teh, {Ye Chean} and Chooi, {Ming Yao} and Dehua Liu and Immanuel Kwok and Lai, {Ghee Chuan} and Yong, {Liyana Ayub Ow} and Melissa Ng and Li, {Jackson L.Y.} and Yingrou Tan and Maximilien Evrard and Leonard Tan and Liong, {Ka Hang} and Keith Leong and Goh, {Chi Ching} and Chan, {Andrew Y.J.} and Shadan, {Nurhidaya Binte} and Mantri, {Chinmay Kumar} and Hwang, {You Yi} and Hui Cheng and Tao Cheng and Weimiao Yu and Tey, {Hong Liang} and Anis Larbi and John, {Ashley St} and Veronique Angeli and Christiane Ruedl and Bernett Lee and Florent Ginhoux and Chen, {Swaine L.} and Ng, {Lai Guan} and Ding, {Jeak Ling} and Chong, {Shu Zhen}",

note = "Publisher Copyright: Copyright {\textcopyright} 2022 The Authors.",

year = "2022",

month = mar,

day = "1",

doi = "10.1126/sciadv.abj4641",

language = "English",

volume = "8",

journal = "Science Advances",

issn = "2375-2548",

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Teh, YC, Chooi, MY, Liu, D, Kwok, I, Lai, GC, Yong, LAO, Ng, M, Li, JLY, Tan, Y, Evrard, M, Tan, L, Liong, KH, Leong, K, Goh, CC, Chan, AYJ, Shadan, NB, Mantri, CK, Hwang, YY, Cheng, H, Cheng, T, Yu, W, Tey, HL, Larbi, A, John, AS, Angeli, V, Ruedl, C, Lee, B, Ginhoux, F, Chen, SL, Ng, LG, Ding, JL & Chong, SZ 2022, 'Transitional premonocytes emerge in the periphery for host defense against bacterial infections', Science Advances, vol. 8, no. 9, eabj4641. https://doi.org/10.1126/sciadv.abj4641

TY - JOUR

T1 - Transitional premonocytes emerge in the periphery for host defense against bacterial infections

AU - Teh, Ye Chean

AU - Chooi, Ming Yao

AU - Liu, Dehua

AU - Kwok, Immanuel

AU - Lai, Ghee Chuan

AU - Yong, Liyana Ayub Ow

AU - Ng, Melissa

AU - Li, Jackson L.Y.

AU - Tan, Yingrou

AU - Evrard, Maximilien

AU - Tan, Leonard

AU - Liong, Ka Hang

AU - Leong, Keith

AU - Goh, Chi Ching

AU - Chan, Andrew Y.J.

AU - Shadan, Nurhidaya Binte

AU - Mantri, Chinmay Kumar

AU - Hwang, You Yi

AU - Cheng, Hui

AU - Cheng, Tao

AU - Yu, Weimiao

AU - Tey, Hong Liang

AU - Larbi, Anis

AU - John, Ashley St

AU - Angeli, Veronique

AU - Ruedl, Christiane

AU - Lee, Bernett

AU - Ginhoux, Florent

AU - Chen, Swaine L.

AU - Ng, Lai Guan

AU - Ding, Jeak Ling

AU - Chong, Shu Zhen

PY - 2022/3/1

Y1 - 2022/3/1

N2 - Circulating Ly6Chi monocytes often undergo cellular death upon exhaustion of their antibacterial effector functions, which limits their capacity for subsequent macrophage differentiation. This shrouds the understanding on how the host replaces the tissue-resident macrophage niche effectively during bacterial invasion to avert infection morbidity. Here, we show that proliferating transitional premonocytes (TpMos), an immediate precursor of mature Ly6Chi monocytes (MatMos), were mobilized into the periphery in response to acute bacterial infection and sepsis. TpMos were less susceptible to apoptosis and served as the main source of macrophage replenishment when MatMos were vulnerable toward bacteria-induced cellular death. Furthermore, TpMo and its derived macrophages contributed to host defense by balancing the proinflammatory cytokine response of MatMos. Consequently, adoptive transfer of TpMos improved the survival outcome of lethal sepsis. Our findings hence highlight a protective role for TpMos during bacterial infections and their contribution toward monocyte-derived macrophage heterogeneity in distinct disease outcomes.

AB - Circulating Ly6Chi monocytes often undergo cellular death upon exhaustion of their antibacterial effector functions, which limits their capacity for subsequent macrophage differentiation. This shrouds the understanding on how the host replaces the tissue-resident macrophage niche effectively during bacterial invasion to avert infection morbidity. Here, we show that proliferating transitional premonocytes (TpMos), an immediate precursor of mature Ly6Chi monocytes (MatMos), were mobilized into the periphery in response to acute bacterial infection and sepsis. TpMos were less susceptible to apoptosis and served as the main source of macrophage replenishment when MatMos were vulnerable toward bacteria-induced cellular death. Furthermore, TpMo and its derived macrophages contributed to host defense by balancing the proinflammatory cytokine response of MatMos. Consequently, adoptive transfer of TpMos improved the survival outcome of lethal sepsis. Our findings hence highlight a protective role for TpMos during bacterial infections and their contribution toward monocyte-derived macrophage heterogeneity in distinct disease outcomes.

UR - http://www.scopus.com/inward/record.url?scp=85125790365&partnerID=8YFLogxK

U2 - 10.1126/sciadv.abj4641

DO - 10.1126/sciadv.abj4641

M3 - Article

C2 - 35245124

AN - SCOPUS:85125790365

SN - 2375-2548

VL - 8

JO - Science Advances

JF - Science Advances

IS - 9

M1 - eabj4641

ER -

Transitional premonocytes emerge in the periphery for host defense against bacterial infections

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