TY - JOUR
T1 - Tubular clathrin/AP-2 lattices pinch collagen fibers to support 3D cell migration
AU - Elkhatib, Nadia
AU - Bresteau, Enzo
AU - Baschieri, Francesco
AU - Rioja, Alba López
AU - Van Niel, Guillaume
AU - Vassilopoulos, Stéphane
AU - Montagnac, Guillaume
N1 - Funding Information:
The authors thank M. Piel, B. Ladoux, P. Chavrier, and J. Ivaska for critical comments on the manuscript. We thank the Gustave Roussy imaging facility for help with image acquisition and the Electron Microscopy Facility of the Institut de Biologie Paris-Seine (IBPS). Core funding for this work was provided by the Gustave Roussy Institute and INSERM, and additional support was provided by grants from ATIP/Avenir Program and la Ligue Nationale Contre le Cancer and from the Agence Nationale de la Recherche (ANR-15-CE15-0005-03) to G.M. and a young researcher grant (ANR-14-CE12-0001-01) to S.V. N.E. designed and performed experiments, analyzed results, and wrote the manuscript. E.B., F.B., and A.L.R. performed experiments. G.V.N. designed and performed electron microscopy analyses in the 3D environment. S.V. designed and performed metal-replica electron microscopy analyses and wrote the manuscript. G.M. supervised the study, designed experiments, and wrote the manuscript. Data described can be found in the main figures and supplementary materials. The authors declare no competing financial interests.
PY - 2017/6/16
Y1 - 2017/6/16
N2 - Migrating cells often use focal adhesions in order to move. Focal adhesions are less prominent in cells migrating in three-dimensional (3D) as compared with 2D environments.We looked for alternative adhesion structures supporting cell migration.We analyzed the dynamics of clathrin-coated pits in cells migrating in a 3D environment of collagen fibers. Both topological cues and local engagement of integrins triggered the accumulation of clathrin-coated structures on fibers. Clathrin/adaptor protein 2 (AP-2) lattices pinched collagen fibers by adopting a tube-like morphology and regulated adhesion to fibers in an endocytosis-independent manner. During migration, tubular clathrin/AP-2 lattices stabilized cellular protrusions by providing anchoring points to collagen fibers. Thus, tubular clathrin/AP-2 lattices promote cell adhesion that, in coordination with focal adhesions, supports cell migration in 3D.
AB - Migrating cells often use focal adhesions in order to move. Focal adhesions are less prominent in cells migrating in three-dimensional (3D) as compared with 2D environments.We looked for alternative adhesion structures supporting cell migration.We analyzed the dynamics of clathrin-coated pits in cells migrating in a 3D environment of collagen fibers. Both topological cues and local engagement of integrins triggered the accumulation of clathrin-coated structures on fibers. Clathrin/adaptor protein 2 (AP-2) lattices pinched collagen fibers by adopting a tube-like morphology and regulated adhesion to fibers in an endocytosis-independent manner. During migration, tubular clathrin/AP-2 lattices stabilized cellular protrusions by providing anchoring points to collagen fibers. Thus, tubular clathrin/AP-2 lattices promote cell adhesion that, in coordination with focal adhesions, supports cell migration in 3D.
UR - http://www.scopus.com/inward/record.url?scp=85020926750&partnerID=8YFLogxK
U2 - 10.1126/science.aal4713
DO - 10.1126/science.aal4713
M3 - Article
C2 - 28619886
AN - SCOPUS:85020926750
SN - 0036-8075
VL - 356
JO - Science
JF - Science
IS - 6343
M1 - 4713
ER -