A semi-automated method for isolating functionally intact mitochondria from cultured cells and tissue biopsies

Sabine Schmitt; Friederike Saathoff; Lilja Meissner; Eva Maria Schropp; Josef Lichtmannegger; Sabine Schulz; Carola Eberhagen; Sabine Borchard; Michaela Aichler; Jerzy Adamski; Nikolaus Plesnila; Simon Rothenfusser; Guido Kroemer; Hans Zischka

doi:10.1016/j.ab.2013.08.007

A semi-automated method for isolating functionally intact mitochondria from cultured cells and tissue biopsies

Sabine Schmitt, Friederike Saathoff, Lilja Meissner, Eva Maria Schropp, Josef Lichtmannegger, Sabine Schulz, Carola Eberhagen, Sabine Borchard, Michaela Aichler, Jerzy Adamski, Nikolaus Plesnila, Simon Rothenfusser, Guido Kroemer, Hans Zischka

Résultats de recherche: Contribution à un journal › Article › Revue par des pairs

49 Citations (Scopus)

Résumé

Mitochondrial dysfunctions decisively contribute to the progression of human diseases, implying that functional tests of isolated mitochondria may furnish conclusive information for diagnosis and therapy. Classical mitochondrial isolation methods, however, lack precisely adjustable settings for cell rupture, which is the most critical step in this procedure, and this complicates subsequent analyses. Here, we present an efficient method to isolate functionally active, intact mitochondria from cultured or primary cells and minute tissue samples in a rapid, highly reproducible manner.

langue originale	Anglais
Pages (de - à)	66-74
Nombre de pages	9
journal	Analytical Biochemistry
Volume	443
Numéro de publication	1
Les DOIs	https://doi.org/10.1016/j.ab.2013.08.007
état	Publié - 1 janv. 2013

Accès au document

10.1016/j.ab.2013.08.007

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Contient cette citation

Schmitt, S., Saathoff, F., Meissner, L., Schropp, E. M., Lichtmannegger, J., Schulz, S., Eberhagen, C., Borchard, S., Aichler, M., Adamski, J., Plesnila, N., Rothenfusser, S., Kroemer, G., & Zischka, H. (2013). A semi-automated method for isolating functionally intact mitochondria from cultured cells and tissue biopsies. Analytical Biochemistry, 443(1), 66-74. https://doi.org/10.1016/j.ab.2013.08.007

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abstract = "Mitochondrial dysfunctions decisively contribute to the progression of human diseases, implying that functional tests of isolated mitochondria may furnish conclusive information for diagnosis and therapy. Classical mitochondrial isolation methods, however, lack precisely adjustable settings for cell rupture, which is the most critical step in this procedure, and this complicates subsequent analyses. Here, we present an efficient method to isolate functionally active, intact mitochondria from cultured or primary cells and minute tissue samples in a rapid, highly reproducible manner.",

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author = "Sabine Schmitt and Friederike Saathoff and Lilja Meissner and Schropp, {Eva Maria} and Josef Lichtmannegger and Sabine Schulz and Carola Eberhagen and Sabine Borchard and Michaela Aichler and Jerzy Adamski and Nikolaus Plesnila and Simon Rothenfusser and Guido Kroemer and Hans Zischka",

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Schmitt, S, Saathoff, F, Meissner, L, Schropp, EM, Lichtmannegger, J, Schulz, S, Eberhagen, C, Borchard, S, Aichler, M, Adamski, J, Plesnila, N, Rothenfusser, S, Kroemer, G & Zischka, H 2013, 'A semi-automated method for isolating functionally intact mitochondria from cultured cells and tissue biopsies', Analytical Biochemistry, VOL. 443, Numéro 1, p. 66-74. https://doi.org/10.1016/j.ab.2013.08.007

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T1 - A semi-automated method for isolating functionally intact mitochondria from cultured cells and tissue biopsies

AU - Schmitt, Sabine

AU - Saathoff, Friederike

AU - Meissner, Lilja

AU - Schropp, Eva Maria

AU - Lichtmannegger, Josef

AU - Schulz, Sabine

AU - Eberhagen, Carola

AU - Borchard, Sabine

AU - Aichler, Michaela

AU - Adamski, Jerzy

AU - Plesnila, Nikolaus

AU - Rothenfusser, Simon

AU - Kroemer, Guido

AU - Zischka, Hans

PY - 2013/1/1

Y1 - 2013/1/1

N2 - Mitochondrial dysfunctions decisively contribute to the progression of human diseases, implying that functional tests of isolated mitochondria may furnish conclusive information for diagnosis and therapy. Classical mitochondrial isolation methods, however, lack precisely adjustable settings for cell rupture, which is the most critical step in this procedure, and this complicates subsequent analyses. Here, we present an efficient method to isolate functionally active, intact mitochondria from cultured or primary cells and minute tissue samples in a rapid, highly reproducible manner.

AB - Mitochondrial dysfunctions decisively contribute to the progression of human diseases, implying that functional tests of isolated mitochondria may furnish conclusive information for diagnosis and therapy. Classical mitochondrial isolation methods, however, lack precisely adjustable settings for cell rupture, which is the most critical step in this procedure, and this complicates subsequent analyses. Here, we present an efficient method to isolate functionally active, intact mitochondria from cultured or primary cells and minute tissue samples in a rapid, highly reproducible manner.

KW - Balch homogenizer

KW - Biopsies

KW - Cell culture

KW - Mitochondria

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DO - 10.1016/j.ab.2013.08.007

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SN - 0003-2697

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SP - 66

EP - 74

JO - Analytical Biochemistry

JF - Analytical Biochemistry

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