AID-dependent somatic hypermutation occurs as a DNA single-strand event in the BL2 cell line

Ahmad Faili, Said Aoufouchi, Quentin Guéranger, Carole Zober, Anne Léon, Barbara Bertocci, Jean Claude Weill, Claude Agnès Reynaud

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    Résumé

    Immunoglobulin (Ig) gene hypermutation can be induced in the BL2 Burkitt's lymphoma cell line by IgM cross-linking and coculture with normal or transformed T helper clones. We describe here a T cell-independent in vitro induction assay, by which hypermutation is induced in BL2 cells through simultaneous aggregation of three surface receptors: IgM, CD19 and CD21. The mutations arise as a post-transcriptional event within 90 min. They are stably introduced in the GI phase of the cell cycle, occurring in one of the two variable gene DNA strands, and eventually become fixed by replication in one of the daughter cells. Inactivation of AID (activation-induced cytidine deaminase) by homologous recombination in BL2 cells completely inhibits the process, thus validating this induction procedure as a model for the in vivo mechanism.

    langue originaleAnglais
    Pages (de - à)815-821
    Nombre de pages7
    journalNature Immunology
    Volume3
    Numéro de publication9
    Les DOIs
    étatPublié - 1 janv. 2002

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