Anti-adenovirus humoral responses influence on the efficacy of vaccines based on epitope display on adenovirus capsid

Anastasia Lanzi; Ghada Ben Youssef; Michel Perricaudet; Karim Benihoud

doi:10.1016/j.vaccine.2010.12.025

Anti-adenovirus humoral responses influence on the efficacy of vaccines based on epitope display on adenovirus capsid

Anastasia Lanzi, Ghada Ben Youssef, Michel Perricaudet, Karim Benihoud

Résultats de recherche: Contribution à un journal › Article › Revue par des pairs

14 Citations (Scopus)

Résumé

The efficacy of recombinant adenoviruses (Ads) vaccine vectors is diminished by the high prevalence of anti-Ad antibodies (Abs) that hampers gene transfer. Epitope display on Ad capsid constitutes an alternative approach to bypass anti-Ad Ab capacity from blocking antigen expression. To understand the role of the epitope insertion site, an ovalbumin-derived epitope was genetically inserted into either Ad hexon or fiber proteins. Hexon-modified Ads triggered higher anti-ovalbumin Ab responses after one injection but surprisingly fiber-modified Ads were by far more potent after two or several administrations. Our data unravel a role for anti-Ad humoral immunity in controlling anti-epitope humoral responses.

langue originale	Anglais
Pages (de - à)	1463-1471
Nombre de pages	9
journal	Vaccine
Volume	29
Numéro de publication	7
Les DOIs	https://doi.org/10.1016/j.vaccine.2010.12.025
état	Publié - 4 févr. 2011

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10.1016/j.vaccine.2010.12.025

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title = "Anti-adenovirus humoral responses influence on the efficacy of vaccines based on epitope display on adenovirus capsid",

abstract = "The efficacy of recombinant adenoviruses (Ads) vaccine vectors is diminished by the high prevalence of anti-Ad antibodies (Abs) that hampers gene transfer. Epitope display on Ad capsid constitutes an alternative approach to bypass anti-Ad Ab capacity from blocking antigen expression. To understand the role of the epitope insertion site, an ovalbumin-derived epitope was genetically inserted into either Ad hexon or fiber proteins. Hexon-modified Ads triggered higher anti-ovalbumin Ab responses after one injection but surprisingly fiber-modified Ads were by far more potent after two or several administrations. Our data unravel a role for anti-Ad humoral immunity in controlling anti-epitope humoral responses.",

keywords = "Adenovirus, Capsid, Epitope",

author = "Anastasia Lanzi and Youssef, {Ghada Ben} and Michel Perricaudet and Karim Benihoud",

note = "Funding Information: We are very grateful to all members of IGR animal facility for technical help. We thank Laetitia Aymeric, Laurence Zitvogel, and Philippe Rameau for providing us with help for cell sorting experiments. This work was supported by the Centre National de la Recherche Scientifique . Anastasia Lanzi received a fellowship from the Minist{\`e}re de la Recherche et de la Technologie. ",

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AU - Lanzi, Anastasia

AU - Youssef, Ghada Ben

AU - Perricaudet, Michel

AU - Benihoud, Karim

N1 - Funding Information: We are very grateful to all members of IGR animal facility for technical help. We thank Laetitia Aymeric, Laurence Zitvogel, and Philippe Rameau for providing us with help for cell sorting experiments. This work was supported by the Centre National de la Recherche Scientifique . Anastasia Lanzi received a fellowship from the Ministère de la Recherche et de la Technologie.

PY - 2011/2/4

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N2 - The efficacy of recombinant adenoviruses (Ads) vaccine vectors is diminished by the high prevalence of anti-Ad antibodies (Abs) that hampers gene transfer. Epitope display on Ad capsid constitutes an alternative approach to bypass anti-Ad Ab capacity from blocking antigen expression. To understand the role of the epitope insertion site, an ovalbumin-derived epitope was genetically inserted into either Ad hexon or fiber proteins. Hexon-modified Ads triggered higher anti-ovalbumin Ab responses after one injection but surprisingly fiber-modified Ads were by far more potent after two or several administrations. Our data unravel a role for anti-Ad humoral immunity in controlling anti-epitope humoral responses.

AB - The efficacy of recombinant adenoviruses (Ads) vaccine vectors is diminished by the high prevalence of anti-Ad antibodies (Abs) that hampers gene transfer. Epitope display on Ad capsid constitutes an alternative approach to bypass anti-Ad Ab capacity from blocking antigen expression. To understand the role of the epitope insertion site, an ovalbumin-derived epitope was genetically inserted into either Ad hexon or fiber proteins. Hexon-modified Ads triggered higher anti-ovalbumin Ab responses after one injection but surprisingly fiber-modified Ads were by far more potent after two or several administrations. Our data unravel a role for anti-Ad humoral immunity in controlling anti-epitope humoral responses.

KW - Adenovirus

KW - Capsid

KW - Epitope

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