TY - JOUR
T1 - Antigenicity and immunogenicity of the C-terminal peptide of human thyroglobulin
AU - El Hassani, Rabii Ameziane
AU - Estienne, Valérie
AU - Blanchin, Stéphanie
AU - Durand-Gorde, Josée Martine
AU - Mallet, Bernard
AU - De Micco, Catherine
AU - Carayon, Pierre
AU - Lalaoui, Khalid
AU - Ruf, Jean
PY - 2004/1/1
Y1 - 2004/1/1
N2 - Thyroglobulin (Tg) is cleaved into several peptides during thyroid hormone synthesis, an oxidative process. P40, an iodinated C-terminal peptide from human Tg, has a molecular weight of about 40 kDa and contains two hormonogenic sites. P40 is the smallest peptide that is still recognized by monoclonal antibodies from mice immunized with human Tg directed against its immunodominant region. Since P40 also contains several T-cell epitopes, it is a good candidate for studying the primary events involved in the process of hormone synthesis leading to thyroid autoimmunity. The present results show that P40 is recognized by Tg antibodies from patients with thyroid disorders and induces Tg antibodies in CBA mice. P40 may therefore be involved in the autoimmune process, thus providing a useful tool for diagnostic and therapeutic purposes.
AB - Thyroglobulin (Tg) is cleaved into several peptides during thyroid hormone synthesis, an oxidative process. P40, an iodinated C-terminal peptide from human Tg, has a molecular weight of about 40 kDa and contains two hormonogenic sites. P40 is the smallest peptide that is still recognized by monoclonal antibodies from mice immunized with human Tg directed against its immunodominant region. Since P40 also contains several T-cell epitopes, it is a good candidate for studying the primary events involved in the process of hormone synthesis leading to thyroid autoimmunity. The present results show that P40 is recognized by Tg antibodies from patients with thyroid disorders and induces Tg antibodies in CBA mice. P40 may therefore be involved in the autoimmune process, thus providing a useful tool for diagnostic and therapeutic purposes.
KW - Autoimmunity
KW - Hormone synthesis
KW - Reactive oxygen species
KW - Thyroglobulin
KW - Thyroid
UR - http://www.scopus.com/inward/record.url?scp=2942620244&partnerID=8YFLogxK
U2 - 10.1016/j.peptides.2004.03.020
DO - 10.1016/j.peptides.2004.03.020
M3 - Article
C2 - 15203250
AN - SCOPUS:2942620244
SN - 0196-9781
VL - 25
SP - 1021
EP - 1029
JO - Peptides
JF - Peptides
IS - 6
ER -