Ase1p organizes antiparallel microtubule arrays during interphase and mitosis in fission yeast

Isabelle Loïodice, Jayme Staub, Thanuja Gangi Setty, Nam Phuong T. Nguyen, Anne Paoletti, P. T. Tran

Résultats de recherche: Contribution à un journalArticleRevue par des pairs

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Résumé

Proper microtubule organization is essential for cellular processes such as organelle positioning during interphase and spindle formation during mitosis. The fission yeast Schizosaccharomyces pombe presents a good model for understanding microtubule organization. We identify fission yeast ase1p, a member of the conserved ASE1/PRC1/MAP65 family of microtubule bundling proteins, which functions in organizing the spindle midzone during mitosis. Using fluorescence live cell imaging, we show that ase1p localizes to sites of microtubule overlaps associated with microtubule organizing centers at both interphase and mitosis. ase1Δ mutants fail to form overlapping antiparallel microtubule bundles, leading to interphase nuclear positioning defects, and premature mitotic spindle collapse. FRAP analysis revealed that interphase ase1p at overlapping microtubule minus ends is highly dynamic. In contrast, mitotic ase1p at microtubule plus ends at the spindle midzone is more stable. We propose that ase1p functions to organize microtubules into overlapping antiparallel bundles both in interphase and mitosis and that ase1p may be differentially regulated through the cell cycle.

langue originaleAnglais
Pages (de - à)1756-1768
Nombre de pages13
journalMolecular Biology of the Cell
Volume16
Numéro de publication4
Les DOIs
étatPublié - 1 avr. 2005
Modification externeOui

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