Assessment of EGFP-Q74 degradation for the measurement of autophagic flux

Yan Wang; Jiao Meng; Qi Wu; Guo Chen; Wei Xie; Zhen Zhang; Guido Kroemer; Oliver Kepp

doi:10.1016/bs.mcb.2020.10.018

Assessment of EGFP-Q74 degradation for the measurement of autophagic flux

Yan Wang, Jiao Meng, Qi Wu, Guo Chen, Wei Xie, Zhen Zhang, Guido Kroemer, Oliver Kepp

Résultats de recherche: Le chapitre dans un livre, un rapport, une anthologie ou une collection › !!Chapter › Revue par des pairs

1 Citation (Scopus)

Résumé

Autophagy plays a major role in physiological and pathological processes. The quantitation of the abundance of autophagy-specific substrates constitutes an efficient strategy for assessing autophagic activity. Here, we provide a detailed protocol for quantifying the decay of a fusion protein composed by enhanced green fluorescent protein (EGFP) and glutamine repeats (Q74) using regular or high-throughput fluorescence microscopy. This method provides a direct measurement of autophagic flux in a Huntington's disease model.

langue originale	Anglais
titre	Monitoring Vesicular Trafficking in Cellular Responses to Stress - Part B
rédacteurs en chef	Oliver Kepp, Lorenzo Galluzzi
Editeur	Academic Press Inc.
Pages	31-38
Nombre de pages	8
ISBN (imprimé)	9780128244876
Les DOIs	https://doi.org/10.1016/bs.mcb.2020.10.018
état	Publié - 1 janv. 2021

Série de publications

Nom	Methods in Cell Biology
Volume	165
ISSN (imprimé)	0091-679X

Accès au document

10.1016/bs.mcb.2020.10.018

Autres fichiers et liens

Lien vers la publication dans Scopus

Contient cette citation

Wang, Y., Meng, J., Wu, Q., Chen, G., Xie, W., Zhang, Z., Kroemer, G., & Kepp, O. (2021). Assessment of EGFP-Q74 degradation for the measurement of autophagic flux. Dans O. Kepp, & L. Galluzzi (eds.), Monitoring Vesicular Trafficking in Cellular Responses to Stress - Part B (p. 31-38). (Methods in Cell Biology; Vol 165). Academic Press Inc.. https://doi.org/10.1016/bs.mcb.2020.10.018

@inbook{4962c1ab27af4d4eb6300301dc0787c0,

title = "Assessment of EGFP-Q74 degradation for the measurement of autophagic flux",

abstract = "Autophagy plays a major role in physiological and pathological processes. The quantitation of the abundance of autophagy-specific substrates constitutes an efficient strategy for assessing autophagic activity. Here, we provide a detailed protocol for quantifying the decay of a fusion protein composed by enhanced green fluorescent protein (EGFP) and glutamine repeats (Q74) using regular or high-throughput fluorescence microscopy. This method provides a direct measurement of autophagic flux in a Huntington's disease model.",

keywords = "Autophagy, EGFP-Q74, Fluorescence microscopy, Huntington's disease",

author = "Yan Wang and Jiao Meng and Qi Wu and Guo Chen and Wei Xie and Zhen Zhang and Guido Kroemer and Oliver Kepp",

note = "Publisher Copyright: {\textcopyright} 2021 Elsevier Inc.",

year = "2021",

month = jan,

day = "1",

doi = "10.1016/bs.mcb.2020.10.018",

language = "English",

isbn = "9780128244876",

series = "Methods in Cell Biology",

publisher = "Academic Press Inc.",

pages = "31--38",

editor = "Oliver Kepp and Lorenzo Galluzzi",

booktitle = "Monitoring Vesicular Trafficking in Cellular Responses to Stress - Part B",

}

Assessment of EGFP-Q74 degradation for the measurement of autophagic flux. / Wang, Yan; Meng, Jiao; Wu, Qi et al.
Monitoring Vesicular Trafficking in Cellular Responses to Stress - Part B. Ed. / Oliver Kepp; Lorenzo Galluzzi. Academic Press Inc., 2021. p. 31-38 (Methods in Cell Biology; Vol 165).

Résultats de recherche: Le chapitre dans un livre, un rapport, une anthologie ou une collection › !!Chapter › Revue par des pairs

TY - CHAP

T1 - Assessment of EGFP-Q74 degradation for the measurement of autophagic flux

AU - Wang, Yan

AU - Meng, Jiao

AU - Wu, Qi

AU - Chen, Guo

AU - Xie, Wei

AU - Zhang, Zhen

AU - Kroemer, Guido

AU - Kepp, Oliver

PY - 2021/1/1

Y1 - 2021/1/1

N2 - Autophagy plays a major role in physiological and pathological processes. The quantitation of the abundance of autophagy-specific substrates constitutes an efficient strategy for assessing autophagic activity. Here, we provide a detailed protocol for quantifying the decay of a fusion protein composed by enhanced green fluorescent protein (EGFP) and glutamine repeats (Q74) using regular or high-throughput fluorescence microscopy. This method provides a direct measurement of autophagic flux in a Huntington's disease model.

AB - Autophagy plays a major role in physiological and pathological processes. The quantitation of the abundance of autophagy-specific substrates constitutes an efficient strategy for assessing autophagic activity. Here, we provide a detailed protocol for quantifying the decay of a fusion protein composed by enhanced green fluorescent protein (EGFP) and glutamine repeats (Q74) using regular or high-throughput fluorescence microscopy. This method provides a direct measurement of autophagic flux in a Huntington's disease model.

KW - Autophagy

KW - EGFP-Q74

KW - Fluorescence microscopy

KW - Huntington's disease

UR - http://www.scopus.com/inward/record.url?scp=85111914980&partnerID=8YFLogxK

U2 - 10.1016/bs.mcb.2020.10.018

DO - 10.1016/bs.mcb.2020.10.018

M3 - Chapter

C2 - 34311869

AN - SCOPUS:85111914980

SN - 9780128244876

T3 - Methods in Cell Biology

SP - 31

EP - 38

BT - Monitoring Vesicular Trafficking in Cellular Responses to Stress - Part B

A2 - Kepp, Oliver

A2 - Galluzzi, Lorenzo

PB - Academic Press Inc.

ER -