Biochemistry: Ubiquitin-binding domains in Y-family polymerases regulate translesion synthesis

Marzena Bienko, Catherine M. Green, Nicola Crosetto, Fabian Rudolf, Grzegorz Zapart, Barry Coull, Patricia Kannouche, Gerhard Wider, Matthias Peter, Alan R. Lehmann, Kay Hofmann, Ivan Dikic

    Résultats de recherche: Contribution à un journalArticleRevue par des pairs

    609 Citations (Scopus)

    Résumé

    Translesion synthesis (TLS) is the major pathway by which mammalian cells replicate across DNA lesions. Upon DNA damage, ubiquitination of proliferating cell nuclear antigen (PCNA) induces bypass of the lesion by directing the replication machinery into the TLS pathway. Yet, how this modification is recognized and interpreted in the cell remains unclear. Here we describe the identification of two ubiquitin (Ub)-binding domains (UBM and UBZ), which are evolutionarily conserved in all Y-family TLS polymerases (pols). These domains are required for binding of polη and poll to ubiquitin, their accumulation in replication factories, and their interaction with monoubiquitinated PCNA. Moreover, the UBZ domain of polη is essential to efficiently restore a normal response to ultraviolet irradiation in xeroderma pigmentosum variant (XP-V) fibroblasts. Our results indicate that Ub-binding domains of Y-family polymerases play crucial regulatory roles in TLS.

    langue originaleAnglais
    Pages (de - à)1821-1824
    Nombre de pages4
    journalScience
    Volume310
    Numéro de publication5755
    Les DOIs
    étatPublié - 16 déc. 2005

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