TY - CHAP
T1 - Biomarker Identification in Liver Cancers Using Desorption Electrospray Ionization Mass Spectrometry (DESI-MS) Imaging
T2 - An Approach for Spatially Resolved Metabolomics
AU - Chen, Hui
AU - Durand, Sylvère
AU - Bawa, Olivia
AU - Bourgin, Mélanie
AU - Montégut, Léa
AU - Lambertucci, Flavia
AU - Motiño, Omar
AU - Li, Sijing
AU - Nogueira-Recalde, Uxía
AU - Anagnostopoulos, Gerasimos
AU - Maiuri, Maria Chiara
AU - Kroemer, Guido
AU - Martins, Isabelle
N1 - Publisher Copyright:
© The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024.
PY - 2024/1/1
Y1 - 2024/1/1
N2 - Liver cancers are characterized by interindividual and intratumoral heterogeneity, which makes early diagnosis and the development of therapies challenging. Desorption electrospray ionization mass spectrometry (DESI-MS) imaging is a potent and sensitive MS ionization technique for direct, unaltered 2D and 3D imaging of metabolites in complex biological samples. Indeed, DESI gently desorbs and ionizes analyte molecules from the sample surface using an electrospray source of highly charged aqueous spray droplets in ambient conditions. DESI-MS imaging of biological samples allows untargeted analysis and characterization of metabolites in liver cancers to identify new biomarkers of malignancy. In this chapter, we described a detailed protocol using liver cancer samples collected and stored for histopathology examination, either as frozen or as formalin-fixed, paraffin-embedded specimens. Such hepatocellular carcinoma samples can be subjected to DESI-MS analyses, illustrating the capacity of spatially resolved metabolomics to distinguish malignant lesions from adjacent normal liver tissue.
AB - Liver cancers are characterized by interindividual and intratumoral heterogeneity, which makes early diagnosis and the development of therapies challenging. Desorption electrospray ionization mass spectrometry (DESI-MS) imaging is a potent and sensitive MS ionization technique for direct, unaltered 2D and 3D imaging of metabolites in complex biological samples. Indeed, DESI gently desorbs and ionizes analyte molecules from the sample surface using an electrospray source of highly charged aqueous spray droplets in ambient conditions. DESI-MS imaging of biological samples allows untargeted analysis and characterization of metabolites in liver cancers to identify new biomarkers of malignancy. In this chapter, we described a detailed protocol using liver cancer samples collected and stored for histopathology examination, either as frozen or as formalin-fixed, paraffin-embedded specimens. Such hepatocellular carcinoma samples can be subjected to DESI-MS analyses, illustrating the capacity of spatially resolved metabolomics to distinguish malignant lesions from adjacent normal liver tissue.
KW - Cancer
KW - DESI-MS; spatial metabolomics
KW - Liver
UR - http://www.scopus.com/inward/record.url?scp=85184401745&partnerID=8YFLogxK
U2 - 10.1007/978-1-0716-3694-7_15
DO - 10.1007/978-1-0716-3694-7_15
M3 - Chapter
C2 - 38315399
AN - SCOPUS:85184401745
T3 - Methods in Molecular Biology
SP - 199
EP - 209
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -