Cell death assays for drug discovery

Oliver Kepp, Lorenzo Galluzzi, Marta Lipinski, Junying Yuan, Guido Kroemer

    Résultats de recherche: Contribution à un journalArticle 'review'Revue par des pairs

    467 Citations (Scopus)

    Résumé

    Cell death has an important role in many human diseases, and strategies aimed at modulating the associated pathways have been successfully applied to treat various disorders. Indeed, several clinically promising cytotoxic and cytoprotective agents with potential applications in cancer, ischaemic and neurodegenerative diseases have recently been identified by high-throughput screening (HTS), based on appropriate cell death assays. Given that different cell death modalities may be dysregulated in different diseases, it is becoming increasingly clear that such assays need to not only quantify the extent of cell death, but they must also be able to distinguish between the various pathways. Here, we systematically describe approaches to accurately quantify distinct cell death pathways, discuss their advantages and pitfalls, and focus on those techniques that are amenable to HTS.

    langue originaleAnglais
    Pages (de - à)221-237
    Nombre de pages17
    journalNature Reviews Drug Discovery
    Volume10
    Numéro de publication3
    Les DOIs
    étatPublié - 1 mars 2011

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