Cellulomonas endometrii sp. nov. a novel bacterium isolated from the endometrial microbiota

Linda Abou Chacra, Marion Bonnet, Mégane Heredia, Gabriel Haddad, Nicholas Armstrong, Stéphane Alibar, Florence Bretelle, Florence Fenollar

Résultats de recherche: Contribution à un journalArticleRevue par des pairs

Résumé

An isolate of a bacterium recovered from an endometrial biopsy failed to be identified by MALDI-TOF mass spectrometry and was subjected to 16S rRNA sequencing. The obtained sequence was compared by BLASTn against the NCBI database, which revealed that the most closely related species was Cellulomonas hominis and Cellulomonas pakistanensis, with 98.85% and 98.45% identity, respectively. Phenotypic characterisation and genome sequencing were performed. The isolate was facultative anaerobic, gram-positive, motile, non-spore forming, and rod-shaped. Cell wall fatty acid profiling revealed that 12-methyl-tetradecanoic acid was the most abundant fatty acid (36%). The genome size was 4.25 Mbp with a G + C content of 74.8 mol%. Genomic comparison of species closely related to this strain showed that all digital DNA–DNA hybridisation (dDDH) and mean orthologous nucleotide identity (OrthoANI) values were below published species thresholds (70% and 95–96%, respectively). Based on these data, we conclude that this isolate represents a new bacterial species belonging to the family Cellulomonadaceae and the phylum Actinomycetota. We propose the name Cellulomonas endometrii sp. nov. The type strain is Marseille-Q7820T (= CSUR Q7820 = CECT 30716).

langue originaleAnglais
Numéro d'article377
journalArchives of Microbiology
Volume205
Numéro de publication12
Les DOIs
étatPublié - 1 nov. 2023
Modification externeOui

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