Characterization of Developmental Trajectories of Dendritic Cell Hematopoiesis Through Single-Cell RNA Sequencing Methods

Regine J. Dress, Florent Ginhoux

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    1 Citation (Scopus)

    Résumé

    Single-cell technologies have become valuable tools to trace dendritic cell differentiation trajectories. Here, we illustrate the workflow used for processing of mouse bone marrow for single-cell RNA sequencing and trajectory analyses, as done in Dress et al. (Nat Immunol 20:852–864, 2019). This short methodology is presented as a starting point for researchers just beginning to dive into the complex field of dendritic cell ontogeny and cellular development trajectory analyses.

    langue originaleAnglais
    titreMethods in Molecular Biology
    EditeurHumana Press Inc.
    Pages375-385
    Nombre de pages11
    Les DOIs
    étatPublié - 1 janv. 2023

    Série de publications

    NomMethods in Molecular Biology
    Volume2618
    ISSN (imprimé)1064-3745
    ISSN (Electronique)1940-6029

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